MEK3+MEK6 Antibody (YA3323)(PBS only)

Cat. No.: HY-P83578A: Data Sheet User Guide for Antibodies
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MEK3+MEK6 Antibody (YA3323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK3+MEK6.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MEK3+MEK6 Antibody (YA3323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK3+MEK6.

Host

Rabbit

Molecular Weight

Predicted band size: 39,37 kDa;

Observed band size: 39,37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P46734 / P52564

Gene ID

5606 [NCBI] / 5608 [NCBI]

Immunogen

A synthesized peptide derived from human MEK3/MEK6

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	200-1:1000
FC FC: Flow Cytometry	1:100-1:200
IP IP: Immunoprecipitation	1:20-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：P46734: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14
P52564: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6.

Subcellular Localization：P52564: Nucleus; Cytoplasm; Cytoplasm, cytoskeleton

Expression：
Tissue_specificity: P46734: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues
P52564: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas
Induction: P52564: Strongly activated by UV, anisomycin, and osmotic shock but not by phorbol esters, NGF or EGF

Subunit：P46734: Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381). Binds to DYRK1B/MIRK and increases its kinase activity (PubMed:11980910). Part of a complex with MAP3K3, RAC1 and CCM2 (By similarity). Interacts with ARRB1 (PubMed:16709866)
P52564: Dimer. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity).

Research Field

Signal Transduction

Synonyms

MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6; MEK3; MAP kinase kinase 3; MAPKK3; MAPK/ERK kinase 3

Documentation

User Guide for Antibodies (1077 KB)