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ERR alpha Antibody (YA2283)

(Synonyms: ESRRA; ERRa; ERR1; HERR1; NR3B1; Steroid hormone receptor ERR1; ERR-alpha; ERRalpha; ESRL1; Estrogen receptor-like 1; Estrogen-related receptor alpha)
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Based on 1 Customer Validation

ERR alpha Antibody (YA2283) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERR alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
  • ERR alpha Antibody (YA2283) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50 1:50-1:100

Product Details

Description

ERR alpha Antibody (YA2283) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERR alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ERR alpha aa1-28.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104643

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ERR alpha Antibody (YA2283)
    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Hela (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ERR alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ERR alpha Antibody (HY-P82538) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ERR alpha binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Induction:Induced by PGC1alpha in a number of specific cell types including heart, kidney and muscle

  • Isoforms & Post-Translational Modification

    P11474 has 2 isomers: P11474-1: 45510 Da (predicted); P11474-2: 45439 Da (predicted).
    Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity;Sumoylated with SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activity, but has no effect on subcellular location nor on DNA binding;Reversibly acetylated. Acetylation by PCAF/KAT2 at Lys-129, Lys-138, Lys-160 and Lys-162 and PCAF/KAT2 decreases transcriptional activity probably by inhibiting DNA-binding activity; deacetylation involves SIRT1 and HDAC8 and increases DNA-binding

  • Subunit

    Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transcriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation. Interacts with MAPK15; promotes re-localization of ESRRA to the cytoplasm through a XPO1-dependent mechanism then inhibits ESRRA transcriptional activity

  • SwissProt ID

    P11474

  • Gene ID
    2101 [NCBI]
  • Synonyms

    ESRRA; ERRa; ERR1; HERR1; NR3B1; Steroid hormone receptor ERR1; ERR-alpha; ERRalpha; ESRL1; Estrogen receptor-like 1; Estrogen-related receptor alpha

  • Research Field

    Epigenetics and Nuclear Signaling

ERR alpha Antibody (YA2283) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Hela (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ERR alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ERR alpha Antibody (HY-P82538) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ERR alpha Antibody (YA2283) Product Image
    1/16
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
  • ERR alpha Antibody (YA2283) Product Image
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Hela (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ERR alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ERR alpha Antibody (HY-P82538) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
  • Experimental Validation Results for ERR alpha Antibody (YA2283)
  • ERR alpha Antibody (YA2283) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Hela (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ERR alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ERR alpha Antibody (YA2283) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ERR alpha Antibody (HY-P82538) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ERR alpha Antibody (YA2283) Product Image WB
  • ERR alpha Antibody (YA2283) Product Image WB