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  5. ATP6V0D1 Antibody (YA2208)

ATP6V0D1 Antibody (YA2208)

Cat. No.: HY-P82463
COA
SDS
User Guide for Antibodies Technical Support

ATP6V0D1 Antibody (YA2208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V0D1.

For research use only. We do not sell to patients.

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50 μL In-stock
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

ATP6V0D1 Antibody (YA2208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V0D1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 40 kDa;
Observed band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human ATP6V0D1 aa1-351.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:200
FC
FC: Flow Cytometry
1:50-1:100
IP
IP: Immunoprecipitation
1:20-1:50
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:28296633, PubMed:30374053, PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:30374053). May play a role in coupling of proton transport and ATP hydrolysis (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity)
Subcellular Localization：Membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Peripheral membrane protein; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein
Expression：
Tissue_specificity:Ubiquitous
Subunit：V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (PubMed:33065002).
Database

Entrez Gene: 9114 Human ; 11972 Mouse ; 291969 Rat

SwissProt: P61421 Human ; P51863 Mouse ;

OMIM: 607028 Human

Research Field

Signal Transduction
Synonyms
P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

ATP6V0D1 Antibody (YA2208) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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