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KDM5C/Jarid1C/SMCX Antibody (YA1063)

(Synonyms: MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E)
Customer Review

Based on 1 Customer Validation

KDM5C/Jarid1C/SMCX Antibody (YA1063) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to KDM5C/Jarid1C/SMCX.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KDM5C/Jarid1C/SMCX Antibody (YA1063)
  • KDM5C/Jarid1C/SMCX Antibody (YA1063) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

KDM5C/Jarid1C/SMCX Antibody (YA1063) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to KDM5C/Jarid1C/SMCX.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 220 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 176 kDa
Species Reactivity Database

Entrez Gene: 8242 Human

SwissProt: P41229 Human

Immunogen

Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KDM5C/Jarid1C/SMCX Antibody (YA1063)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and A431 (lane 4, 15 μg) using KDM5C Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    KDM5C/Jarid1C/SMCX is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2[1].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It was expressed in all tissues tested. The highest levels were found in the brain and skeletal muscle.

  • Isoforms & Post-Translational Modification

    P41229 has 5 isomers: P41229-1: 175720 Da (predicted); P41229-2: 175379 Da (predicted); P41229-3: 170561 Da (predicted); P41229-4: 155119 Da (predicted); P41229-5: 175592 Da (predicted).

  • Subunit

    Part of two distinct complexes, one containing E2F6, and the other containing REST (PubMed:17468742). Interacts with ZMYND8 (PubMed:33323928)

  • SwissProt ID

    P41229

  • Gene ID
    8242 [NCBI]
  • Synonyms

    MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E

  • Research Field

    Epigenetics and Nuclear Signaling

KDM5C/Jarid1C/SMCX Antibody (YA1063) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and A431 (lane 4, 15 μg) using KDM5C Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • KDM5C/Jarid1C/SMCX Antibody (YA1063) Product Image
    1/16
  • Experimental Validation Results for KDM5C/Jarid1C/SMCX Antibody (YA1063)
  • KDM5C/Jarid1C/SMCX Antibody (YA1063) Product Image
  • Experimental Validation Results for KDM5C/Jarid1C/SMCX Antibody (YA1063)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and A431 (lane 4, 15 μg) using KDM5C Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for KDM5C/Jarid1C/SMCX Antibody (YA1063)
  • KDM5C/Jarid1C/SMCX Antibody (YA1063) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and A431 (lane 4, 15 μg) using KDM5C Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • KDM5C/Jarid1C/SMCX Antibody (YA1063) Product Image WB