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ENT2 Antibody (YA10002)

(Synonyms: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8)

ENT2 Antibody (YA10002) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to ENT2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ENT2 Antibody (YA10002)
  • ENT2 Antibody (YA10002) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-200 1:50-200

Product Details

Description

ENT2 Antibody (YA10002) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to ENT2.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55-65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Synthetic peptide corresponding to human ENT2.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ENT2 Antibody (YA10002)
    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), K562 (lane 3), SH-SY5Y (lane 4), and MCF-7 (lane 5) using ENT2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    ENT2 is a Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent, passive transporter. Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine. Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Also mediates nicotinamide uptake supporting homeostasis and modulating its biological activities. Can also transport urate. Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites. Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier. Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity)[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Apical cell membrane; Basolateral cell membrane; Cell membrane

  • Expression


    Tissue_Specificity: Highly expressed in skeletal muscle. Expressed in liver, lung, placenta, brain, heart, kidney and ovarian tissues. Expressed in testis at the blood-brain-barrier.

  • Isoforms & Post-Translational Modification

    ENT2 has 4 isoforms, Q14542-1: amino acid length is 456, molecular weight is 50113 Da (predicted); Q14542-2: amino acid length is 202, molecular weight is 22276 Da (predicted); Q14542-3: amino acid length is 301, molecular weight is 32507 Da (predicted); Q14542-4: amino acid length is 361, molecular weight is 38758 Da (predicted).Glycosylated

  • SwissProt ID

    Q14542

  • Gene ID
    3177 [NCBI]
  • Synonyms

    DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8

[1]. Ward JL, et al. Kinetic and pharmacological properties of cloned human equilibrative nucleoside transporters, ENT1 and ENT2, stably expressed in nucleoside transporter-deficient PK15 cells. Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine. J Biol Chem. 2000 Mar 24;275(12):8375-81. [Content Brief]

[2]. Mangravite LM, et al. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am J Physiol Renal Physiol. 2003 May;284(5):F902-10. [Content Brief]

[3]. Ward JL, et al. Functional analysis of site-directed glycosylation mutants of the human equilibrative nucleoside transporter-2. Arch Biochem Biophys. 2003 Mar 1;411(1):19-26. [Content Brief]

[4]. Owen RP, et al. Functional characterization and haplotype analysis of polymorphisms in the human equilibrative nucleoside transporter, ENT2. Drug Metab Dispos. 2006 Jan;34(1):12-5. [Content Brief]

[5]. Yao SY, et al. Nucleobase transport by human equilibrative nucleoside transporter 1 (hENT1). J Biol Chem. 2011 Sep 16;286(37):32552-62. [Content Brief]

[6]. Griffiths M, et al. Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta. Biochem J. 1997 Dec 15;328 ( Pt 3)(Pt 3):739-43. [Content Brief]

[7]. Crawford CR, et al. Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line. J Biol Chem. 1998 Feb 27;273(9):5288-93. [Content Brief]

[8]. Chen M, et al. SLC29A1 and SLC29A2 are human nicotinamide cell membrane transporters. Nat Commun. 2025 Jan 30;16(1):1181. [Content Brief]

[9]. Matake I, et al. Functional characteristics of equilibrative nucleoside transporter 2 (ENT2/SLC29A2) for the transport of urate as a newly identified substrate. Drug Metab Pharmacokinet. 2025 Jun;62:101048. [Content Brief]

[10]. Klein DM, et al. Basolateral uptake of nucleosides by Sertoli cells is mediated primarily by equilibrative nucleoside transporter 1. J Pharmacol Exp Ther. 2013 Jul;346(1):121-9. [Content Brief]

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ENT2 Antibody (YA10002) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), K562 (lane 3), SH-SY5Y (lane 4), and MCF-7 (lane 5) using ENT2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ENT2 Antibody (YA10002) Product Image
    1/16
  • Experimental Validation Results for ENT2 Antibody (YA10002)
  • ENT2 Antibody (YA10002) Product Image
  • Experimental Validation Results for ENT2 Antibody (YA10002)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), K562 (lane 3), SH-SY5Y (lane 4), and MCF-7 (lane 5) using ENT2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ENT2 Antibody (YA10002)
  • ENT2 Antibody (YA10002) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), K562 (lane 3), SH-SY5Y (lane 4), and MCF-7 (lane 5) using ENT2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ENT2 Antibody (YA10002) Product Image WB