RNF144A Antibody
(Synonyms: KIAA0161, RNF144, UBCE7IP4, RNF144A, E3 ubiquitin-protein ligase RNF144A, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4)RNF144A Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RNF144A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
RNF144A Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RNF144A.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32 kDa
Synthetic peptide corresponding to the center region of human RNF144A.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RNF144A is an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC during DNA damage. Positively regulates DNA virus or exogenous cytosolic DNA-triggered innate immune response by mediating STING1 ubiquitination and increasing its 'Lys-6'-linked ubiquitination and translocation from the endoplasmic reticulum to the Golgi leading to downstream signaling pathways. Plays a positive role in EGF-dependent cell proliferation by prolonging EGF/EGFR signaling during EGF stimulation through EGFR ubiquitination. Increases ERK activity independently of EGFR signaling by promoting polyubiquitination and subsequent degradation of VRK3 in the cytosol[1][2][3][4][5].
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Subcellular Localization
Cell membrane; Cytoplasmic vesicle membrane; Endosome membrane; Endoplasmic reticulum membrane
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Isoforms & Post-Translational Modification
RNF144A has an amino acid length of 292, molecular weight is 32890 Da.
Auto-ubiquitinated. -
Subunit
Self-associates.
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SwissProt ID
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Synonyms
KIAA0161, RNF144, UBCE7IP4, RNF144A, E3 ubiquitin-protein ligase RNF144A, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4
Documentation
References
[1]. Ho SR, et al. Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain. J Biol Chem. 2015 Sep 18;290(38):23026-38. [Content Brief]
[2]. Ho SR, et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2646-55. [Content Brief]
[3]. Yang B, et al. RNF144A promotes antiviral responses by modulating STING ubiquitination. EMBO Rep. 2023 Dec 6;24(12):e57528. [Content Brief]
[4]. Ho SR, et al. RNF144A sustains EGFR signaling to promote EGF-dependent cell proliferation. J Biol Chem. 2018 Oct 19;293(42):16307-16323. [Content Brief]
[5]. Han SH, et al. RNF144a induces ERK-dependent cell death under oxidative stress via downregulation of vaccinia-related kinase 3. J Cell Sci. 2020 Nov 9;133(21):. [Content Brief]
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