NUDT21 Antibody (YA7844)(PBS only)
(Synonyms: CFIM25; CPSF5)NUDT21 Antibody (YA7844) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NUDT21.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:150-500 | 1:100-250 |
Product Details
NUDT21 Antibody (YA7844) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NUDT21.
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Host Mouse
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 26 kDa
Human recombinant protein fragment corresponding to amino acids 2-227 of human NUDT21 produced in E.coli.
Affinity purified
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NUDT21 component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides[1][2][3][4][5][6][7][8][9][10][11][12][13][14].
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Subcellular Localization
Nucleus,Cytoplasm
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Expression
Tissue_Specificity: Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas -
Isoforms & Post-Translational Modification
O43809: 227 amino acids, molecular weight 26227 Da.
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Subunit
Homodimer (via N- and C-terminus); binds RNA as homodimer (PubMed:18445629, PubMed:20479262, PubMed:20695905)
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SwissProt ID
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Synonyms
CFIM25; CPSF5
Documentation
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