RES Antibody (YA9764)
(Synonyms: REST, RE1 Silencing Transcription Factor, NRSF, XBR, RE1-Silencing Transcription Factor, Neural-Restrictive Silencer Factor, DFNA27, Neuron-Restrictive Silencer Factor, Neuron Restrictive Silencer Factor, Deafness, Autosomal Dominant 27, Repressor Binding To The X2 Box, X2 Box Repressor, GINGF5, HGF5, WT6)RES Antibody (YA9764) is a Mouse-derived and non-conjugated IgG2a Monoclonal antibody, targeting to RES.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, mouse, rat
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Formulation:
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:400-2000 | 1:100-1000 |
Product Details
RES Antibody (YA9764) is a Mouse-derived and non-conjugated IgG2a Monoclonal antibody, targeting to RES.
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Host Mouse
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Species ReactivityHuman, mouse, rat
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Observed Molecular WeightObserved band size: 122 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 122 kDa
Recombinant protein of human REST
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RES is a Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (By similarity). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 3 (By similarity). Repressor activity may be inhibited by forming heterodimers with isoform 3, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes. Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes (By similarity). Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons. Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits (By similarity). Acts as a regulator of osteoblast differentiation (By similarity). Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions. May also function in stress resistance in the brain during aging; possibly by regulating expression of genes involved in cell death and in the stress response. Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death (By similarity). After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death (By similarity). Negatively regulates the expression of SRRM3 in breast cancer cell lines[1][2][3][4][5][6][7][8][9][10][11][12][13][14].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_Specificity: Expressed in neurons of the prefrontal cortex, in hippocampal pyramidal neurons, dentate gyrus granule neurons and cerebellar Purkinje and granule neurons (at protein level). Expressed in dopaminergic neurons of the substantia nigra (at protein level). Expressed in neural progenitor cells (at protein level). In patients suffering from Alzheimer disease, frontotemporal dementia or dementia with Lewy bodies, decreased nuclear levels have been observed in neurons of the prefrontal cortex and the hippocampus, but not in neurons of the dentate gyrus and cerebellum (at protein level). In patients with Parkinson disease or dementia with Lewy bodies, decreased nuclear levels have been observed in dopaminergic neurons and in cortical neurons and localization to Lewy bodies and pale bodies was detected (at protein level). Expressed at higher levels in weakly invasive breast cancer cell lines and at lower levels in highly invasive breast cancer lines (at protein level). Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
Induction: Up-regulated by Wnt signaling. Up-regulated in the brain of aging individuals but not in Alzheimer disease patients. Up-regulated by oxidative stress. Down-regulated during neural progenitor cell differentiation. -
Isoforms & Post-Translational Modification
RES has 4 isoforms, Q13127-1: amino acid length is 1097, molecular weight is 121872 Da (predicted); Q13127-2: amino acid length is 313, molecular weight is 35048 Da (predicted); Q13127-3: amino acid length is 329, molecular weight is 36972 Da (predicted); Q13127-4: amino acid length is 1074, molecular weight is 119083 Da (predicted).O-glycosylated
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Subunit
Isoform 1 and isoform 3 form heterodimers (By similarity).
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SwissProt ID
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Synonyms
REST, RE1 Silencing Transcription Factor, NRSF, XBR, RE1-Silencing Transcription Factor, Neural-Restrictive Silencer Factor, DFNA27, Neuron-Restrictive Silencer Factor, Neuron Restrictive Silencer Factor, Deafness, Autosomal Dominant 27, Repressor Binding To The X2 Box, X2 Box Repressor, GINGF5, HGF5, WT6
Documentation
[1]. Magin A, et al. RE-1 silencing transcription factor-4 (REST4) is neither a transcriptional repressor nor a de-repressor. Neurochem Int. 2002 Mar;40(3):195-202. [Content Brief]
[2]. Tabuchi A, et al. REST4-mediated modulation of REST/NRSF-silencing function during BDNF gene promoter activation. Biochem Biophys Res Commun. 2002 Jan 11;290(1):415-20. [Content Brief]
[3]. Lunyak VV, et al. Corepressor-dependent silencing of chromosomal regions encoding neuronal genes. Science. 2002 Nov 29;298(5599):1747-52. [Content Brief]
[4]. Mahamdallie SS, et al. Mutations in the transcriptional repressor REST predispose to Wilms tumor. Nat Genet. 2015 Dec;47(12):1471-4. [Content Brief]
[5]. Chong JA, et al. REST: a mammalian silencer protein that restricts sodium channel gene expression to neurons. Cell. 1995 Mar 24;80(6):949-57. [Content Brief]
[6]. Schoenherr CJ, et al. The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes. Science. 1995 Mar 3;267(5202):1360-3. [Content Brief]
[7]. Scholl T, et al. A zinc finger protein that represses transcription of the human MHC class II gene, DPA. J Immunol. 1996 Feb 15;156(4):1448-57. [Content Brief]
[8]. Andrés ME, et al. CoREST: a functional corepressor required for regulation of neural-specific gene expression. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9873-8. [Content Brief]
[9]. Grimes JA, et al. The co-repressor mSin3A is a functional component of the REST-CoREST repressor complex. J Biol Chem. 2000 Mar 31;275(13):9461-7. [Content Brief]
[10]. Mulligan P, et al. CDYL bridges REST and histone methyltransferases for gene repression and suppression of cellular transformation. Mol Cell. 2008 Dec 5;32(5):718-26. [Content Brief]
[11]. Huang Z, et al. Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural progenitor cells. Nat Cell Biol. 2011 Feb;13(2):142-52. [Content Brief]
[12]. Cavadas MA, et al. REST is a hypoxia-responsive transcriptional repressor. Sci Rep. 2016 Aug 17;6:31355. [Content Brief]
[13]. Lu T, et al. REST and stress resistance in ageing and Alzheimer's disease. Nature. 2014 Mar 27;507(7493):448-54. [Content Brief]
[14]. Lee NS, et al. Non-coding RNAs derived from an alternatively spliced REST transcript (REST-003) regulate breast cancer invasiveness. Sci Rep. 2015 Jun 8;5:11207. [Content Brief]
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