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c-Fos Antibody (YA506)

(Synonyms: G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

c-Fos Antibody (YA506) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to c-Fos.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • c-Fos Antibody (YA506) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500 1:50-1:200 1:100

Product Details

Description

c-Fos Antibody (YA506) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to c-Fos.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 41/55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human c-Fos.AA range:231-268.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102070

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for c-Fos Antibody (YA506)
    Western blot analysis of extracts from Hela(lane 2(20μg) , C6 (lane 3(20μg) ,HEK293T(lane 4(20μg)and MCF-7( lane 5(20μg) using c-Fos Antibody (HY-P80081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    Immunohistochemical analysis of paraffin-embedded human ‌Cervical Carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    c-Fos is an immediate-early gene product and transcription factor that functions as a central mediator of stimulus-dependent gene expression, linking transient extracellular signals to long-term cellular responses through regulation of downstream target genes[1]. Mechanistically, c-Fos belongs to the Fos family and forms the activator protein-1 (AP-1) transcription factor complex through dimerization with Jun family proteins, thereby regulating genes involved in cellular differentiation, proliferation, development, plasticity, and survival[4][3][2]. c-Fos expression is rapidly induced by neuronal activity, calcium- and cAMP-dependent signaling, and other environmental stimuli, making it a widely used molecular marker of cellular activation and stimulus-transcription coupling in experimental systems[1][3]. In disease and experimental models, c-Fos has been extensively employed to map activated neuronal populations during seizures, learning, memory formation, and behavioral responses, providing a framework for investigating neural circuit function and activity-dependent transcriptional programs[1][3]. Compared with related Fos family members, including FosB, Fra-1 (FOSL1), and Fra-2 (FOSL2), c-Fos contains a C-terminal transactivation domain and exhibits distinctive transcriptional regulatory properties within AP-1 complexes[4]. For experimental applications, c-Fos is primarily used as an activity-dependent biomarker rather than a direct pharmacological target, enabling quantitative assessment of cellular activation, neural network recruitment, and stimulus-responsive signaling pathways in neuroscience and molecular biology research[3][5].

  • Subcellular Localization

    Nucleus; Endoplasmic reticulum; Cytoplasm, cytosol

  • Subunit

    Heterodimer; with JUN (By similarity). Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1 promoter site (PubMed:9732876). Interacts with SMAD3; the interaction is weak even on TGF-beta activation (PubMed:9732876). Interacts with MAFB (By similarity). Interacts with TSC22D3 (via N-terminus); this interaction inhibits the binding of active AP1 to its target DNA (By similarity). Interacts with CDS1 and PI4K2A (By similarity). Interacts (via bZIP domain and leucine-zipper region) with the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF) subunits SMARCB1, SMARCC2 and SMARCD1 (By similarity). Interacts (via bZIP domain and leucine-zipper region) with ARID1A (By similarity)

  • SwissProt ID

    P01100

  • Gene ID
    2353 [NCBI]
  • Synonyms

    G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

  • Research Field

    Neuroscience

[1]. Gallo FT, et al. Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Front Behav Neurosci. 2018;12:79.

[2]. Curran T, et al. Fos: an immediate-early transcription factor in neurons. J Neurobiol. 1995 Mar;26(3):403-12. [Content Brief]

[3]. Lara Aparicio SY, et al. Current Opinion on the Use of c-Fos in Neuroscience. NeuroSci. 2022;3(4):687-702.

[4]. Srivastava SK, et al. Genetic regulation of spy gene expression in Escherichia coli in the presence of protein unfolding agent ethanol. Gene. 2014 Sep 10;548(1):142-8. [Content Brief]

[5]. Cruz-Mendoza F, et al. Immediate early gene c-fos in the brain: focus on glial cells. Brain Sci. 2022;12(6):687. [Content Brief]

[6]. Lara Aparicio SY, et al. Current opinion on the use of c-Fos in neuroscience. NeuroSci. 2022;3(4):687-702. [Content Brief]

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c-Fos Antibody (YA506) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , C6 (lane 3(20μg) ,HEK293T(lane 4(20μg)and MCF-7( lane 5(20μg) using c-Fos Antibody (HY-P80081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Cervical Carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • c-Fos Antibody (YA506) Product Image
    1/16
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • c-Fos Antibody (YA506) Product Image
  • Experimental Validation Results for c-Fos Antibody (YA506)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , C6 (lane 3(20μg) ,HEK293T(lane 4(20μg)and MCF-7( lane 5(20μg) using c-Fos Antibody (HY-P80081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Cervical Carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • Experimental Validation Results for c-Fos Antibody (YA506)
  • c-Fos Antibody (YA506) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) , C6 (lane 3(20μg) ,HEK293T(lane 4(20μg)and MCF-7( lane 5(20μg) using c-Fos Antibody (HY-P80081). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • c-Fos Antibody (YA506) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • c-Fos Antibody (YA506) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Cervical Carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • c-Fos Antibody (YA506) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • c-Fos Antibody (YA506) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • c-Fos Antibody (YA506) Product Image WB
  • c-Fos Antibody (YA506) Product Image IHC-P
  • c-Fos Antibody (YA506) Product Image IHC-P
  • c-Fos Antibody (YA506) Product Image mIHC
  • c-Fos Antibody (YA506) Product Image mIHC