Bik Antibody (YA6887)

Cat. No.: HY-P87199: Data Sheet COA
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User Guide for Antibodies
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Bik Antibody (YA6887) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bik.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

Bik Antibody (YA6887) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bik.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size：18 kDa;

Observed band size: 18 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13323

Immunogen

Recombinant protein within human Bik aa 1-160.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Raji (lane 1(20μg)) 、Ramos (lane 2(20μg)) 、THP-1 (lane 3(20μg)) and SW480 (lane 4(20μg)) using Bik Antibody (HY-P87199) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/10000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX

Subcellular Localization：Endomembrane system,Mitochondrion membrane

Isoforms & Post-Translational Modification：Q13323: 160 amino acids, molecular weight 18016 Da.

Subunit：Interacts with RHBDL4/RHBDD1 (PubMed:18953687)

Synonyms

Apoptosis inducer NBK antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]