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CDCA3 Antibody (YA3176)

(Synonyms: Cell division cycle-associated protein 3; Trigger of mitotic entry protein 1; TOME-1; Gene-rich cluster protein C8; CDCA3; C8; GRCC8; TOME1)
Customer Review

Based on 1 Customer Validation

CDCA3 Antibody (YA3176) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDCA3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    1.Supplied in rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
    2.Supplied in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • CDCA3 Antibody (YA3176) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:50-1:200

Product Details

Description

CDCA3 Antibody (YA3176) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDCA3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database

Entrez Gene: 83461 Human

SwissProt: Q99618 Human ;

Immunogen

A synthesized peptide derived from human CDCA3 aa1-42.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    1.Supplied in rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
    2.Supplied in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDCA3 Antibody (YA3176)
    Western blot analysis of extracts from L929 (lane 2(20μg), A549 (lane 3(20μg), SiHa (lane 4(20μg), HepG2 (lane 5(20μg) using CDCA3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
    Immunocytochemistry analysis of HepG2 cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
    Immunocytochemistry analysis of Hela cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CDCA3 is a F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase

  • Subcellular Localization

    Cytoplasm, cytosol

  • Subunit

    Interacts with SKP1.

  • SwissProt ID

    Q99618

  • Gene ID
    83461 [NCBI]
  • Synonyms

    Cell division cycle-associated protein 3; Trigger of mitotic entry protein 1; TOME-1; Gene-rich cluster protein C8; CDCA3; C8; GRCC8; TOME1

  • Research Field

    Cell Biology

CDCA3 Antibody (YA3176) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from L929 (lane 2(20μg), A549 (lane 3(20μg), SiHa (lane 4(20μg), HepG2 (lane 5(20μg) using CDCA3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDCA3 Antibody (YA3176) Product Image
    1/16
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • CDCA3 Antibody (YA3176) Product Image
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from L929 (lane 2(20μg), A549 (lane 3(20μg), SiHa (lane 4(20μg), HepG2 (lane 5(20μg) using CDCA3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • Experimental Validation Results for CDCA3 Antibody (YA3176)
  • CDCA3 Antibody (YA3176) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from L929 (lane 2(20μg), A549 (lane 3(20μg), SiHa (lane 4(20μg), HepG2 (lane 5(20μg) using CDCA3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CDCA3 Antibody (YA3176) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDCA3 Antibody (YA3176) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CDCA3 Antibody with CDCA3 Antibody (YA3176) (HY-P83431) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CDCA3 Antibody (YA3176) ((HY-P83431) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDCA3 Antibody (YA3176) Product Image WB
  • CDCA3 Antibody (YA3176) Product Image ICC
  • CDCA3 Antibody (YA3176) Product Image ICC