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MD2 Antibody (YA6875)

(Synonyms: ESOP 1 antibody; LY 96 antibody; md 2 antibody)
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Based on 1 Customer Validation

MD2 Antibody (YA6875) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MD2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MD2 Antibody (YA6875)
  • Experimental Validation Results for MD2 Antibody (YA6875)
  • MD2 Antibody (YA6875) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

MD2 Antibody (YA6875) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MD2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：18 kDa
Immunogen

Recombinant protein within human MD2 aa 1-160.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719286

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MD2 Antibody (YA6875)
    Western blot analysis of extracts from Jurkat (lane 1(20μg)) and Mouse testis (lane 2(20μg)) using MD2 Antibody (HY-P87187) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MD2 Antibody (YA6875)
    Western blot analysis was performed on protein extracts (30 μg) from Mouse liver (lane 2), Rat liver (lane 3), Mouse spleen (lane 4), and Mouse thymus (lane 5) using MD2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    MD2 binds bacterial lipopolysaccharide (LPS). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS[1][2][3][4][5].

  • Subcellular Localization

    Secreted, extracellular space,Secreted

  • Isoforms & Post-Translational Modification

    Q9Y6Y9 has two isomers: Q9Y6Y9-1: 18546 Da (predicted); Q9Y6Y9-2: 14933 Da (predicted).
    N-glycosylated; high-mannose

  • Subunit

    Heterogeneous homomer formed from homodimers; disulfide-linked (PubMed:11593030, PubMed:12642668)

  • SwissProt ID

    Q9Y6Y9

  • Synonyms

    ESOP 1 antibody; LY 96 antibody; md 2 antibody

MD2 Antibody (YA6875) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1(20μg)) and Mouse testis (lane 2(20μg)) using MD2 Antibody (HY-P87187) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Mouse liver (lane 2), Rat liver (lane 3), Mouse spleen (lane 4), and Mouse thymus (lane 5) using MD2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MD2 Antibody (YA6875) Product Image
    1/16
  • Experimental Validation Results for MD2 Antibody (YA6875)
  • Experimental Validation Results for MD2 Antibody (YA6875)
  • MD2 Antibody (YA6875) Product Image
  • Experimental Validation Results for MD2 Antibody (YA6875)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1(20μg)) and Mouse testis (lane 2(20μg)) using MD2 Antibody (HY-P87187) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MD2 Antibody (YA6875)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Mouse liver (lane 2), Rat liver (lane 3), Mouse spleen (lane 4), and Mouse thymus (lane 5) using MD2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MD2 Antibody (YA6875)
  • Experimental Validation Results for MD2 Antibody (YA6875)
  • MD2 Antibody (YA6875) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 1(20μg)) and Mouse testis (lane 2(20μg)) using MD2 Antibody (HY-P87187) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • MD2 Antibody (YA6875) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Mouse liver (lane 2), Rat liver (lane 3), Mouse spleen (lane 4), and Mouse thymus (lane 5) using MD2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MD2 Antibody (YA6875) Product Image WB
  • MD2 Antibody (YA6875) Product Image WB