ENPP3 Antibody (YA9706)
(Synonyms: Alkaline phosphodiesterase I, ARHR2, B10, CD203c, CD203c antigen, dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3), dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3), E NPP 3, E-NPP 3, Ectonucleotide pyrophosphatase/phosphodiesterase 3)
Based on 1 Customer Validation
ENPP3 Antibody (YA9706) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to ENPP3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100 | 1:200-1000 |
Product Details
ENPP3 Antibody (YA9706) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to ENPP3.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 100 kDa;
Recombinant protein of human ENPP3 (aa 526-875).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ENPP3 is a Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Also hydrolyzes extracellular 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production, and is therefore involved in the regulation of innate immune response. 2',3'-cGAMP appears to be further processed to GMP, AMP and inorganic phosphate. Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well. In addition, displays an alkaline phosphodiesterase activity in vitro[1][2][3][4][5].
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Subcellular Localization
Cell membrane; Apical cell membrane; Secreted
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Expression
Tissue_Specificity: Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.
Induction: Up-regulated by stimulation by allergen or by cross-linking with IgE. The IgE-mediated activation is enhanced by tetradecanoyl phorbol acetate (TPA), a stimulator of the PKC pathway, and inhibited by the P13 kinase inhibitors, LY294002 and wortmannin. Up-regulated in invasive bile duct cancers. -
Isoforms & Post-Translational Modification
ENPP3 has an amino acid length of 875, molecular weight is 100124 Da.
N-glycosylated. N-glycosylation is necessary for normal transport to the cell membrane, but is not the apical targeting signal. -
Subunit
Monomer and homodimer
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SwissProt ID
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Synonyms
Alkaline phosphodiesterase I, ARHR2, B10, CD203c, CD203c antigen, dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3), dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3), E NPP 3, E-NPP 3, Ectonucleotide pyrophosphatase/phosphodiesterase 3
Documentation
[1]. Gorelik A, et al. Structural basis for nucleotide recognition by the ectoenzyme CD203c. FEBS J. 2018 Jul;285(13):2481-2494. [Content Brief]
[2]. Jin-Hua P, et al. Molecular cloning and chromosomal localization of PD-Ibeta (PDNP3), a new member of the human phosphodiesterase I genes. Genomics. 1997 Oct 15;45(2):412-5. [Content Brief]
[3]. Mardjuki R, et al. Identification of the extracellular membrane protein ENPP3 as a major cGAMP hydrolase and innate immune checkpoint. Cell Rep. 2024 May 28;43(5):114209. [Content Brief]
[4]. Vollmayer P, et al. Hydrolysis of diadenosine polyphosphates by nucleotide pyrophosphatases/phosphodiesterases. Eur J Biochem. 2003 Jul;270(14):2971-8. [Content Brief]
[5]. Bühring HJ, et al. The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3. Blood. 2001 May 15;97(10):3303-5. [Content Brief]
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