Collagen IV Antibody (YA6331)
(Synonyms: Arresten; BSVD; CO4A1_HUMAN; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1; IV; chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Of Basement Membrane Alpha 1 Chain; Collagen Of Basement Membrane Alpha 2 Chain; Collagen Type IV Alpha 1; collagen type IV alpha 1 chain; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RatOR)Collagen IV Antibody (YA6331) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen IV.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:1000-5000 | 1:200-1000 | 1:5000-20000 | 1:50-200 |
Product Details
Collagen IV Antibody (YA6331) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen IV.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 200 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 160 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Collagen IV forms a basement membrane scaffold that maintains matrix integrity under mechanical demand while early basement membrane deposition can proceed without the major α1 (IV) 2α2 (IV) isoform[1]. Mechanistically, collagen IV integrates laminins, nidogens, and perlecan into supramolecular basement membrane architecture, therefore supporting structural barriers and cell-matrix interactions[1]. Six α chains, α1 (IV) to α6 (IV), assemble into three characteristic heterotrimers, creating isoform-specific basement membrane networks rather than one uniform collagen IV molecule[2]. In developing mouse eyes, α1 (IV) and α2 (IV) showed ubiquitous co-localization, whereas α3 (IV) and α4 (IV) showed more spatially and temporally restricted co-localization[2]. Disease relevance follows this isoform logic because defects in type IV collagen are implicated in Alport syndrome and Goodpasture syndrome[3]. In experimental models, Col4a1/2- embryos reached E9.5 but died between E10.5 and E11.5 after basement membrane structural failure[1]. Compared with structural isoforms, collagen IV-derived NC1 fragments provide functional research tools: arresten, canstatin, and tumstatin act as type IV collagen-derived angiogenesis inhibitors[4]. Tumstatin, the α3 (IV) NC1 domain, induces apoptosis of proliferating endothelial cells and suppresses pathological angiogenesis and tumor growth[5]. - Key distinction: collagen IV isoforms differ by α-chain composition, tissue distribution, and disease relevance. - Research application: NC1-derived fragments support angiogenesis, tumor-growth, and biomarker-focused experimental designs.
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Subcellular Localization
Secreted, extracellular space, extracellular matrix, basement membrane
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Expression
Tissue_specificity:High expression in the placenta -
Isoforms & Post-Translational Modification
P02462 has 2 isomers: P02462-1: 160611 Da (predicted); P02462-2: 50155 Da (predicted).
Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated. The modified lysines can be O-glycosylated;Contains 4-hydroxyproline (Probable). Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains (By similarity);Contains 3-hydroxyproline. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline;Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding (PubMed:2844531). 12 of these, located in the NC1 domain, are conserved in all known type IV collagens;The trimeric structure of the NC1 domains is stabilized by covalent bonds (sulfilimine cross-links) between Lys and Met residues (PubMed:12011424). These cross-links are important for the mechanical stability of the basement membrane (By similarity). Sulfilimine cross-link is catalyzed by PXDN (By similarity);Proteolytic processing produces the C-terminal NC1 peptide, arresten -
Subunit
There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. Interacts with EFEMP2 (By similarity)
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SwissProt ID
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Synonyms
Arresten; BSVD; CO4A1_HUMAN; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1; IV; chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Of Basement Membrane Alpha 1 Chain; Collagen Of Basement Membrane Alpha 2 Chain; Collagen Type IV Alpha 1; collagen type IV alpha 1 chain; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RatOR
Documentation
References
[1]. Pöschl E, et al. Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. Development. 2004 Apr;131(7):1619-28. [Content Brief]
[2]. Bai X, et al. Developmental distribution of collagen IV isoforms and relevance to ocular diseases. Matrix Biol. 2009 May;28(4):194-201. [Content Brief]
[3]. Hudson BG, et al. Alport's syndrome, Goodpasture's syndrome, and type IV collagen. N Engl J Med. 2003 Jun 19;348(25):2543-56. [Content Brief]
[4]. Mundel TM, et al. Type IV collagen-derived angiogenesis inhibitors. Microvasc Res. 2007 Sep-Nov;74(2-3):85-9. [Content Brief]
[5]. Hamano Y, et al. Tumstatin, the NC1 domain of alpha3 chain of type IV collagen, is an endogenous inhibitor of pathological angiogenesis and suppresses tumor growth. Biochem Biophys Res Commun. 2005 Jul 29;333(2):292-8. [Content Brief]
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