Copy Link Print Information

LAMP1 Antibody (YA3755)

(Synonyms: LAMPA; CD107a; LGP120)
Customer Review

Based on 1 Customer Validation

LAMP1 Antibody (YA3755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LAMP1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LAMP1 Antibody (YA3755)
  • LAMP1 Antibody (YA3755) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

LAMP1 Antibody (YA3755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LAMP1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey
  • Observed Molecular Weight
    Observed band size: 45/110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Immunogen

Purified recombinant fragment of human LAMP1 (AA: 182-414) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718823

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LAMP1 Antibody (YA3755)
    Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HepG2 (lane 4(20μg), HUVEC (lane 5(20μg) using LAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    LAMP1 is a Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins; (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Also promotes fusion of the virus with host membrane in less acidic endosomes; (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Lysosome membrane; Single-pass type I membrane protein; Endosome membrane; Single-pass type I membrane protein; Late endosome membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cytolytic granule membrane; Single-pass type I membrane protein

  • Isoforms & Post-Translational Modification

    P11279 has 2 isomers: P11279-1: 44882 Da (predicted); P11279-2: 38986 Da (predicted).
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans;(Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells

  • Subunit

    Interacts with ABCB9; this interaction strongly stabilizes ABCB9 and protects ABCB9 against lysosomal degradation (PubMed:22641697). Interacts with FURIN (PubMed:32295904). Interacts with TMEM175; inhibiting the proton channel activity of TMEM175 (PubMed:37390818)

  • SwissProt ID

    P11279

  • Gene ID
    3916 [NCBI]
  • Synonyms

    LAMPA; CD107a; LGP120

LAMP1 Antibody (YA3755) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HepG2 (lane 4(20μg), HUVEC (lane 5(20μg) using LAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LAMP1 Antibody (YA3755) Product Image
    1/16
  • Experimental Validation Results for LAMP1 Antibody (YA3755)
  • LAMP1 Antibody (YA3755) Product Image
  • Experimental Validation Results for LAMP1 Antibody (YA3755)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HepG2 (lane 4(20μg), HUVEC (lane 5(20μg) using LAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LAMP1 Antibody (YA3755)
  • LAMP1 Antibody (YA3755) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HepG2 (lane 4(20μg), HUVEC (lane 5(20μg) using LAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LAMP1 Antibody (YA3755) Product Image WB