LAMP1 Antibody (YA3755)

製品番号: HY-P84058: データシート SDS COA User Guide for Antibodies
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LAMP1 Antibody (YA3755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LAMP1.

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容量	価格（税別）	在庫状況	数量
20 μL	$144	在庫あり	Estimated Time of Arrival: December 31
50 μL	$210	在庫あり	Estimated Time of Arrival: December 31
100 μL	$340	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

LAMP1 Antibody (YA3755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LAMP1.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 45 kDa;

Observed band size: 45/110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

P11279

Gene ID

3916 [NCBI]

Immunogen

Purified recombinant fragment of human LAMP1 (AA: 182-414) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

純度	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), Jurkat (lane 3(20μg), HepG2 (lane 4(20μg), HUVEC (lane 5(20μg) using LAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349); (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909); (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F

Subcellular Localization：Lysosome membrane; Single-pass type I membrane protein; Endosome membrane; Single-pass type I membrane protein; Late endosome membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cytolytic granule membrane; Single-pass type I membrane protein

Isoforms & Post-Translational Modification：P11279 has 2 isomers: P11279-1: 44882 Da (predicted); P11279-2: 38986 Da (predicted).
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans;(Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells

Subunit：Interacts with ABCB9; this interaction strongly stabilizes ABCB9 and protects ABCB9 against lysosomal degradation (PubMed:22641697). Interacts with FURIN (PubMed:32295904). Interacts with TMEM175; inhibiting the proton channel activity of TMEM175 (PubMed:37390818)

RRID

AB_3718823

Synonyms

LAMPA; CD107a; LGP120

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/rpmi-8226-cell.html [Content Brief]