Copy Link Print Information

OTX2 Antibody (YA3956)

(Synonyms: MCOPS5; MGC45000)
Customer Review

Based on 1 Customer Validation

OTX2 Antibody (YA3956) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OTX2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Rabbit, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • OTX2 Antibody (YA3956) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

OTX2 Antibody (YA3956) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OTX2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Rabbit, Monkey
  • Observed Molecular Weight
    Observed band size: 31-36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Immunogen

Purified recombinant fragment of human OTX2 aa 40-297.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718869

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for OTX2 Antibody (YA3956)
    Western blot analysis of extracts from HepG2 (lane2(20μg), U251 (lane3(20μg), Mouse eye tissue (lane4(20μg) and Rat eye tissue (lane5(20μg) using OTX2 Antibody (HY-P84259). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for OTX2 Antibody (YA3956)
    Immunocytochemistry analysis of MCF-7 cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for OTX2 Antibody (YA3956)
    Immunocytochemistry analysis of U2OS cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    OTX2 transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P32243 has 2 isomers: P32243-1: 31636 Da (predicted); P32243-2: 32406 Da (predicted).

  • SwissProt ID

    P32243

  • Gene ID
    5015 [NCBI]
  • Synonyms

    MCOPS5; MGC45000

OTX2 Antibody (YA3956) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), U251 (lane3(20μg), Mouse eye tissue (lane4(20μg) and Rat eye tissue (lane5(20μg) using OTX2 Antibody (HY-P84259). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • OTX2 Antibody (YA3956) Product Image
    1/16
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • OTX2 Antibody (YA3956) Product Image
  • Experimental Validation Results for OTX2 Antibody (YA3956)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), U251 (lane3(20μg), Mouse eye tissue (lane4(20μg) and Rat eye tissue (lane5(20μg) using OTX2 Antibody (HY-P84259). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for OTX2 Antibody (YA3956)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for OTX2 Antibody (YA3956)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • Experimental Validation Results for OTX2 Antibody (YA3956)
  • OTX2 Antibody (YA3956) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane2(20μg), U251 (lane3(20μg), Mouse eye tissue (lane4(20μg) and Rat eye tissue (lane5(20μg) using OTX2 Antibody (HY-P84259). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • OTX2 Antibody (YA3956) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • OTX2 Antibody (YA3956) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling OTX2 with OTX2 Antibody (HY-P84259) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OTX2 Antibody (HY-P84259) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • OTX2 Antibody (YA3956) Product Image WB
  • OTX2 Antibody (YA3956) Product Image ICC
  • OTX2 Antibody (YA3956) Product Image ICC