OTX2 Antibody (YA3956)

Cat. No.: HY-P84259: Ficha de datos COA
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OTX2 Antibody (YA3956) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OTX2.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn
Referencias

Descripciòn

OTX2 Antibody (YA3956) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OTX2.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 32 kDa;

Observed band size: 31-36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit, Monkey

SwissProt ID

P32243

Gene ID

5015 [NCBI]

Immunogen

Purified recombinant fragment of human OTX2 aa 40-297.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2 (lane2(20μg), U251 (lane3(20μg), Mouse eye tissue (lane4(20μg) and Rat eye tissue (lane5(20μg) using OTX2 Antibody (HY-P84259). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P32243 has 2 isomers: P32243-1: 31636 Da (predicted); P32243-2: 32406 Da (predicted).

RRID

AB_3718869

Synonyms

MCOPS5; MGC45000

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/t47d-cell.html [Content Brief]