Copy Link Print Information

CD276/B7H3 Antibody (YA4779)

(Synonyms: CD276; B7H3; PSEC0249; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD antigen CD276)

CD276/B7H3 Antibody (YA4779) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD276/B7H3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • CD276/B7H3 Antibody (YA4779) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000 1:50-200

Product Details

Description

CD276/B7H3 Antibody (YA4779) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD276/B7H3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Purified recombinant fragment of human CD276 expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    CD276/B7-H3 is a member of the B7 family of immune checkpoint molecules and plays an important role in regulating immune responses. As a co-stimulatory/co-inhibitory protein, it affects both adaptive and innate immune responses. CD276/B7-H3 was first discovered on dendritic cells and is widely expressed in various tumor tissues, including breast cancer, lung cancer, ovarian cancer, and gastric cancer. Its upregulation is often associated with poor prognosis, increased tumor invasiveness, and metastasis.
    CD276/B7-H3 has a dual role in the immune system. It can act as a co-stimulatory molecule, enhancing T cell proliferation and cytotoxicity in certain cases, but it can also function as a co-inhibitory molecule by suppressing T cell activity and promoting immune evasion by tumors, thus contributing to tumor progression and metastasis. This dual function makes CD276/B7-H3 a promising target in cancer immunotherapy research. In the tumor microenvironment, its expression is linked to immune suppression, further promoting tumor growth and metastasis.
    Research strategies targeting B7-H3 include monoclonal antibodies, antibody-drug conjugates (ADC), bispecific antibodies, and chimeric antigen receptor (CAR) T cell therapies. These strategies have demonstrated promising results in preclinical studies, enhancing immune responses and inhibiting tumor growth in various tumor models[1].

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is ubiquitous but undetectable in peripheral blood lymphocytes or granulocytes. It is weakly expressed in resting monocytes. It is expressed in monocyte-derived dendritic cells. It is expressed in sinus epithelial cells. It is expressed in extravillous trophoblast cells and Hofbauer cells of the placenta in early pregnancy and at term.

    Induction:By bacterial lipopolysaccharides (LPS) in monocytes and by ionomycin in T and B-lymphocytes. Up-regulated in cells mediating rejection of human transplants

  • Isoforms & Post-Translational Modification

    Q5ZPR3 has 4 isomers: Q5ZPR3-1: 57235 Da (predicted); Q5ZPR3-2: 33791 Da (predicted); Q5ZPR3-3: 52761 Da (predicted); Q5ZPR3-4: 57165 Da (predicted).

  • Subunit

    Interacts with TREML2 and this interaction enhances T-cell activation

  • SwissProt ID

    Q5ZPR3

  • Gene ID
    80381 [NCBI]
  • Synonyms

    CD276; B7H3; PSEC0249; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD antigen CD276

[1]. Zhou WT, et al. B7-H3/CD276: An Emerging Cancer Immunotherapy. Front Immunol. 2021 Jul 19;12:701006. [Content Brief]

CD276/B7H3 Antibody (YA4779) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • CD276/B7H3 Antibody (YA4779) Product Image
    1/16
  • CD276/B7H3 Antibody (YA4779) Product Image