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Cleaved-Caspase 8 Antibody (YA792)

(Synonyms: CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Cleaved-Caspase 8 Antibody (YA792) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cleaved-Caspase 8.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Cleaved-Caspase 8 Antibody (YA792) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

Cleaved-Caspase 8 Antibody (YA792) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cleaved-Caspase 8.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 18, 43-55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43-55 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Caspase-8.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102148

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Jurkat (lane 4(20μg) using Cleaved-Caspase 8 (HY-P80624) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    Western blot analysis of extracts from K562 (lane 2(40μg), 3T3 (lane 3(40μg), MCF-7 (lane 4(40μg)using Cleaved-Caspase 8 Antibody (YA792). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed:16916640, PubMed:8962078, PubMed:9006941). Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A (PubMed:8681376, PubMed:8681377). The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed:9184224). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed:9184224). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed:9184224). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed:31827280, PubMed:31827281). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis (PubMed:32929201, PubMed:34012073). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (PubMed:8755496). Cleaves PARP1 and PARP2 (PubMed:8681376). Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380 (PubMed:18216014, PubMed:27109099); Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex; Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex; Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex (Probable). Acts as an inhibitor of the caspase cascade (PubMed:12010809); Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex

  • Subcellular Localization

    Cytoplasm; Nucleus; Cell projection, lamellipodium

  • Expression


    Tissue_specificity:Isomers 1, 5, and 7 are expressed in a variety of tissues. They are most highly expressed in peripheral blood leukocytes, spleen, thymus, and liver. They are almost undetectable in the brain, testes, and skeletal muscle.

  • Isoforms & Post-Translational Modification

    Q14790 has 9 isomers: Q14790-1: 55391 Da (predicted); Q14790-2: 53768 Da (predicted); Q14790-3: 45929 Da (predicted); Q14790-4: 57701 Da (predicted); Q14790-5: 27484 Da (predicted); Q14790-6: 25862 Da (predicted); Q14790-7: 32330 Da (predicted); Q14790-8: 30707 Da (predicted); Q14790-9: 61836 Da (predicted).
    Generation of the p10 and p18 subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events;Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis (PubMed:20937773). Phosphorylation on Tyr-380 by SRC is mediated by interaction with the SRC SH2 domain and does not affect dimerization or recruitment to the death-inducing signaling complex (DISC) but negatively regulates DISC-mediated processing and activation of CASP8, down-regulating its proapoptotic function (PubMed:16619028, PubMed:27109099). Phosphorylation on Tyr-380 also enhances localization to lamellipodia in migrating cells (PubMed:18216014);(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP8 processing, preventing CASP8 activation and ability to mediate extrinsic apoptosis;(Microbial infection) Proteolytically cleaved by the cowpox virus CRMA death inhibitory protein

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit (PubMed:10508784). Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95 or TNFRSF1A, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (PubMed:8681376, PubMed:8681377, PubMed:9184224). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity). Interacts with CFLAR and PEA15 (PubMed:10442631). Interacts with TNFAIP8L2 (By similarity). Interacts with CASP8AP2 (PubMed:16378960). Interacts with RFFL and RNF34; negatively regulate CASP8 through proteasomal degradation (PubMed:15069192). Interacts with NOL3; decreases CASP8 activity in a mitochondria localization- and phosphorylation-dependent manner and this interaction is dissociated by calcium (PubMed:15509781). Interacts with UBR2ca (PubMed:28602583). Interacts with RIPK1 (By similarity). Interacts with stimulated TNFRSF10B; this interaction is followed by CASP8 proteolytic cleavage and activation (PubMed:18846110). Interacts (phosphorylated on Tyr-380) with PIK3R1 (PubMed:27109099)

  • SwissProt ID

    Q14790

  • Gene ID
    841 [NCBI]
  • Synonyms

    CASP8; MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH

  • Research Field

    Cell Biology

Cleaved-Caspase 8 Antibody (YA792) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Jurkat (lane 4(20μg) using Cleaved-Caspase 8 (HY-P80624) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(40μg), 3T3 (lane 3(40μg), MCF-7 (lane 4(40μg)using Cleaved-Caspase 8 Antibody (YA792). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    1/16
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Cleaved-Caspase 8 Antibody (YA792) Product Image
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Jurkat (lane 4(20μg) using Cleaved-Caspase 8 (HY-P80624) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(40μg), 3T3 (lane 3(40μg), MCF-7 (lane 4(40μg)using Cleaved-Caspase 8 Antibody (YA792). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Experimental Validation Results for Cleaved-Caspase 8 Antibody (YA792)
  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Jurkat (lane 4(20μg) using Cleaved-Caspase 8 (HY-P80624) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(40μg), 3T3 (lane 3(40μg), MCF-7 (lane 4(40μg)using Cleaved-Caspase 8 Antibody (YA792). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Cleaved-Caspase 8 with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cleaved-Caspase 8 Antibody (HY-P80624) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cleaved-Caspase 8 Antibody (YA792) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Cleaved-Caspase 8 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80624, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cleaved-Caspase 8 Antibody (YA792) Product Image WB
  • Cleaved-Caspase 8 Antibody (YA792) Product Image WB
  • Cleaved-Caspase 8 Antibody (YA792) Product Image ICC
  • Cleaved-Caspase 8 Antibody (YA792) Product Image ICC
  • Cleaved-Caspase 8 Antibody (YA792) Product Image IHC-P
  • Cleaved-Caspase 8 Antibody (YA792) Product Image IHC-P