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PRDX3 Antibody (YA909)

(Synonyms: PRDX3; AOP1; Thioredoxin-dependent peroxide reductase; mitochondrial; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog)
Customer Review

Based on 1 Customer Validation

PRDX3 Antibody (YA909) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRDX3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC FC
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • PRDX3 Antibody (YA909) Product Image
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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

PRDX3 Antibody (YA909) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRDX3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database

Entrez Gene: 10935 Human

SwissProt: P30048 Human

Immunogen

Synthetic peptide within Human Peroxiredoxin 3 aa 207-256 / 256.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103185

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRDX3 Antibody (YA909)
    Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using PRDX3(HY-P81066) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    Immunocytochemistry analysis of Hela cells labeling PRDX3 with PRDX3 Antibody (HY-P81066)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    Immunocytochemistry analysis of HepG2 cells labeling PRDX3 with PRDX3 Antibody (HY-P81066) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling PRDX3 Antibody (YA909) (HY-P810669, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    PRDX3 is a Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Required for the maintenance of physical strength[1][2][3][4][5].

  • Subcellular Localization

    Mitochondrion; Cytoplasm; Early endosome

  • Isoforms & Post-Translational Modification

    P30048 has 2 isomers: P30048-1: 27693 Da (predicted); P30048-2: 25839 Da (predicted).
    Phosphorylated by LRRK2; phosphorylation reduces perodixase activity;The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond;S-palmitoylated

  • Subunit

    Homodimer; disulfide-linked, upon oxidation (PubMed:17707404, PubMed:27238969, PubMed:29438714). 6 homodimers assemble to form a ring-like dodecamer (PubMed:17707404, PubMed:27238969, PubMed:29438714). Interacts with NEK6 (PubMed:20873783). Interacts with LRRK2 (PubMed:21850687). Interacts with MAP3K13 (PubMed:12492477). Interacts with RPS6KC1 (via PX domain) (PubMed:15750338)

  • SwissProt ID

    P30048

  • Gene ID
    10935 [NCBI]
  • Synonyms

    PRDX3; AOP1; Thioredoxin-dependent peroxide reductase; mitochondrial; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog

  • Research Field

    Neuroscience

References

[1]. Cao Z, et al. Reconstitution of the mitochondrial PrxIII antioxidant defence pathway: general properties and factors affecting PrxIII activity and oligomeric state. J Mol Biol. 2007 Sep 28;372(4):1022-1033. [Content Brief]

[2]. Yewdall NA, et al. Quaternary structure influences the peroxidase activity of peroxiredoxin 3. Biochem Biophys Res Commun. 2018 Mar 4;497(2):558-563. [Content Brief]

[3]. Rebelo AP, et al. Biallelic loss-of-function variations in PRDX3 cause cerebellar ataxia. Brain. 2021 Jun 22;144(5):1467-1481. [Content Brief]

[4]. Tsuji K, et al. Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli. Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):377-81. [Content Brief]

[5]. Masaki M, et al. Mixed lineage kinase LZK and antioxidant protein-1 activate NF-kappaB synergistically. Eur J Biochem. 2003 Jan;270(1):76-83. [Content Brief]

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PRDX3 Antibody (YA909) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using PRDX3(HY-P81066) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PRDX3 with PRDX3 Antibody (HY-P81066)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PRDX3 with PRDX3 Antibody (HY-P81066) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling PRDX3 Antibody (YA909) (HY-P810669, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PRDX3 Antibody (YA909) Product Image
    1/16
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • PRDX3 Antibody (YA909) Product Image
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using PRDX3(HY-P81066) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PRDX3 with PRDX3 Antibody (HY-P81066)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling PRDX3 with PRDX3 Antibody (HY-P81066) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PRDX3 Antibody (YA909)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling PRDX3 Antibody (YA909) (HY-P810669, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • Experimental Validation Results for PRDX3 Antibody (YA909)
  • PRDX3 Antibody (YA909) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using PRDX3(HY-P81066) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRDX3 Antibody (YA909) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PRDX3 with PRDX3 Antibody (HY-P81066)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control.

  • PRDX3 Antibody (YA909) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling PRDX3 with PRDX3 Antibody (HY-P81066) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PRDX3 Antibody (HY-P81066) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PRDX3 Antibody (YA909) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling PRDX3 Antibody (YA909) (HY-P810669, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PRDX3 Antibody (YA909) Product Image WB
  • PRDX3 Antibody (YA909) Product Image ICC
  • PRDX3 Antibody (YA909) Product Image ICC
  • PRDX3 Antibody (YA909) Product Image FC