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SCXA Antibody (YA7074)

(Synonyms: Basic helix loop helix transcription factor scleraxis antibody; Basic helix-loop-helix transcription factor scleraxis antibody; bHLHa41 antibody; bHLHa48 antibody; Class A basic helix-loop-helix protein 41 antibody; Class A basic helix-loop-helix protein 48 antibody; scleraxis antibody; scleraxis homolog A antibody; SCX antibody; SCX_HUMAN antibody)
Customer Review

Based on 1 Customer Validation

SCXA Antibody (YA7074) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SCXA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SCXA Antibody (YA7074)
  • Experimental Validation Results for SCXA Antibody (YA7074)
  • SCXA Antibody (YA7074) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:100

Product Details

Description

SCXA Antibody (YA7074) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SCXA.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Immunogen

Recombinant protein within human SCXA aa 1-201 / 201.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SCXA Antibody (YA7074)
    Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SCXA Antibody (YA7074)
    Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SCXA plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q7RTU7: 201 amino acids, molecular weight 21596 Da.

  • Subunit

    Efficient DNA binding requires dimerization with another bHLH protein

  • SwissProt ID

    Q7RTU7

  • Synonyms

    Basic helix loop helix transcription factor scleraxis antibody; Basic helix-loop-helix transcription factor scleraxis antibody; bHLHa41 antibody; bHLHa48 antibody; Class A basic helix-loop-helix protein 41 antibody; Class A basic helix-loop-helix protein 48 antibody; scleraxis antibody; scleraxis homolog A antibody; SCX antibody; SCX_HUMAN antibody

SCXA Antibody (YA7074) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SCXA Antibody (YA7074) Product Image
    1/16
  • Experimental Validation Results for SCXA Antibody (YA7074)
  • Experimental Validation Results for SCXA Antibody (YA7074)
  • SCXA Antibody (YA7074) Product Image
  • Experimental Validation Results for SCXA Antibody (YA7074)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SCXA Antibody (YA7074)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SCXA Antibody (YA7074)
  • Experimental Validation Results for SCXA Antibody (YA7074)
  • SCXA Antibody (YA7074) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SCXA Antibody (YA7074) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling SCXA With SCXA antibody (HY-P87391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SCXA antibody (HY-P87391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SCXA Antibody (YA7074) Product Image ICC
  • SCXA Antibody (YA7074) Product Image ICC