Wilms Tumor Protein Antibody (YA2277)(PBS only)

Cat. No.: HY-P82532A: Data Sheet User Guide for Antibodies
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Wilms Tumor Protein Antibody (YA2277) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wilms Tumor Protein.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Wilms Tumor Protein Antibody (YA2277) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wilms Tumor Protein.

Host

Rabbit

Molecular Weight

Predicted band size: 49 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P19544

Gene ID

7490 [NCBI]

Immunogen

A synthesized peptide derived from human Wilms Tumor Protein

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Purity	Affinity Chromatography	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921)

Subcellular Localization：Nucleus; Nucleus, nucleolus; Cytoplasm; Nucleus speckle; Nucleus, nucleoplasm

Expression：
Tissue_specificity:It is expressed in the kidneys and some hematopoietic cells.

Isoforms & Post-Translational Modification：P19544 has 8 isomers: P19544-1: 49188 Da (predicted); P19544-2: 47195 Da (predicted); P19544-3: 47511 Da (predicted); P19544-4: 48872 Da (predicted); P19544-6: 34447 Da (predicted); P19544-7: 56883 Da (predicted); P19544-8: 55206 Da (predicted); P19544-9: 33086 Da (predicted).

Subunit：Homodimer. Interacts with WTIP. Interacts with actively translating polysomes. Detected in nuclear ribonucleoprotein (mRNP) particles. Interacts with HNRNPU via the zinc-finger region. Interacts with U2AF2. Interacts with CITED2 (By similarity). Interacts with ZNF224 via the zinc-finger region. Interacts with WTAP and SRY. Interacts with AMER1. Interacts with RBM4

Research Field

Tags & Cell Markers

Synonyms

GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; WIT 2 antibody; WT 1 antibody; FWT1 antibody; GUD antibody; NPHS4 antibody; WAGR antibody; Wilms tumor 1 antibody; Wilms Tumor antibody; Wilms tumor protein antibody; Wilms' tumor gene antibody; WIT2 antibody; WT antibody; WT1 antibody; WT1_HUMAN antibody; WT33 antibody

Documentation

User Guide for Antibodies (1077 KB)