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Myosin heavy chain Antibody (YA279)

(Synonyms: cardiac muscle alpha isoform antibody; MYH6 antibody; MYH6_HUMAN antibody; MYH7 antibody; MYHC antibody; MyHC-alpha antibody; MyHC-beta antibody; MYHCA antibody; MYHCB antibody; Myosin heavy chain 6 antibody; Myosin heavy chain antibody; Myosin heavy chain cardiac muscle alpha isoform antibody; Myosin heavy chain cardiac muscle beta isoform antibody; Myosin-6 antibody)
Customer Review

Based on 1 Customer Validation

Myosin heavy chain Antibody (YA279) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin heavy chain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Myosin heavy chain Antibody (YA279) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-1:2000 1:100-1:500 1:100

Product Details

Description

Myosin heavy chain Antibody (YA279) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin heavy chain.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 223 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 224 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Myosin heavy chain(MYH6) AA. range:1220-1420.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102367

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Western blot analysis of extracts from Rat heart tissue (lane2, 20μg) and Rat heart tissue (lane3, 40μg) using Myosin heavy chain Antibody (HY-P80234). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    Immunohistochemical analysis of paraffin-embedded human Skeletal muscle tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Myosin heavy chain is a Muscle contraction

  • Subcellular Localization

    Cytoplasm, myofibril

  • Subunit

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2)

  • SwissProt ID

    P13533

  • Gene ID
    4625 [NCBI]
  • Synonyms

    cardiac muscle alpha isoform antibody; MYH6 antibody; MYH6_HUMAN antibody; MYH7 antibody; MYHC antibody; MyHC-alpha antibody; MyHC-beta antibody; MYHCA antibody; MYHCB antibody; Myosin heavy chain 6 antibody; Myosin heavy chain antibody; Myosin heavy chain cardiac muscle alpha isoform antibody; Myosin heavy chain cardiac muscle beta isoform antibody; Myosin-6 antibody

  • Research Field

    Signal Transduction

Myosin heavy chain Antibody (YA279) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat heart tissue (lane2, 20μg) and Rat heart tissue (lane3, 40μg) using Myosin heavy chain Antibody (HY-P80234). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Skeletal muscle tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA279) Product Image
    1/16
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Myosin heavy chain Antibody (YA279) Product Image
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat heart tissue (lane2, 20μg) and Rat heart tissue (lane3, 40μg) using Myosin heavy chain Antibody (HY-P80234). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Skeletal muscle tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA279)
  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat heart tissue (lane2, 20μg) and Rat heart tissue (lane3, 40μg) using Myosin heavy chain Antibody (HY-P80234). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Myocardium tissue using Myosin heavy chain antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80234, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA279) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Skeletal muscle tissue using Myosin heavy chain Antibody(HY-P80234, 1/300) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA279) Product Image WB
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P
  • Myosin heavy chain Antibody (YA279) Product Image IHC-P