alpha 1 Antitrypsin Antibody (YA3591)(PBS only)
(Synonyms: PI; A1A; AAT; PI1; A1AT; SERPINA1)alpha 1 Antitrypsin Antibody (YA3591) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to alpha 1 Antitrypsin.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:100-1:1000 | 1:10000 |
Product Details
alpha 1 Antitrypsin Antibody (YA3591) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to alpha 1 Antitrypsin.
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Host Mouse
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
Purified recombinant fragment of human AAT aa 40-184.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
alpha 1 Antitrypsin is an Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin; Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE)
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Subcellular Localization
Secreted; Endoplasmic reticulum; Secreted, extracellular space, extracellular matrix
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Expression
Tissue_specificity:It is ubiquitous. It is expressed in both leukocytes and plasma. -
Isoforms & Post-Translational Modification
P01009 has 3 isomers: P01009-1: 46737 Da (predicted); P01009-2: 40263 Da (predicted); P01009-3: 34755 Da (predicted).
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant;Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418;(Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases -
Subunit
Interacts with CELA2A (PubMed:31358993). Interacts with ERGIC3 and LMAN1/ERGIC53 (PubMed:31142615). Interacts with PRSS1/trypsin (PubMed:11057674). Interacts with PRSS1/Trypsin (PubMed:11057674). The variants S and Z interact with CANX and PDIA3 (PubMed:23826168)
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SwissProt ID
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Synonyms
PI; A1A; AAT; PI1; A1AT; SERPINA1
Documentation
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