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ARL13B Antibody (YA6956)

(Synonyms: ADP ribosylation factor like 13B antibody; ADP ribosylation factor like 2 like 1 antibody; ADP-ribosylation factor-like protein 13B antibody; ADP-ribosylation factor-like protein 2-like 1 antibody; AR13B_HUMAN antibody; arl13b antibody; ARL2-like protein 1 antibody; ARL2L1 antibody; JBTS8 antibody)
Customer Review

Based on 1 Customer Validation

ARL13B Antibody (YA6956) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ARL13B.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ARL13B Antibody (YA6956)
  • ARL13B Antibody (YA6956) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:2000

Product Details

Description

ARL13B Antibody (YA6956) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ARL13B.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 49 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Immunogen

Synthetic peptide within human ARL13B aa 370-428.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719302

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ARL13B Antibody (YA6956)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HL-60 (lane 2(20μg)) 、HepG2 (lane 3(20μg)) and K562 (lane 4(20μg)) using ARL13B Antibody (HY-P87273) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ARL13B is a Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze fit; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. Plays a role in ciliar trafficking of phosphatidylinositol phosphatase INPP5E in ciliogenesis. May regulate ARF6- and RAB22A-dependent endocytic recycling traffic[1][2].

  • Subcellular Localization

    Cell projection, cilium membrane,Cell projection, cilium,Cell membrane,Cytoplasm, cytoskeleton

  • Expression


    Tissue_Specificity: Expressed in the developing brain

  • Isoforms & Post-Translational Modification

    Q3SXY8 has three isomers: Q3SXY8-1: 48643 Da (predicted); Q3SXY8-2: 36795 Da (predicted); Q3SXY8-3: 37087 Da (predicted).
    Sumoylation is required for PKD2 entry into cilium

  • Subunit

    Monomer (By similarity)

  • SwissProt ID

    Q3SXY8

  • Synonyms

    ADP ribosylation factor like 13B antibody; ADP ribosylation factor like 2 like 1 antibody; ADP-ribosylation factor-like protein 13B antibody; ADP-ribosylation factor-like protein 2-like 1 antibody; AR13B_HUMAN antibody; arl13b antibody; ARL2-like protein 1 antibody; ARL2L1 antibody; JBTS8 antibody

ARL13B Antibody (YA6956) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HL-60 (lane 2(20μg)) 、HepG2 (lane 3(20μg)) and K562 (lane 4(20μg)) using ARL13B Antibody (HY-P87273) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ARL13B Antibody (YA6956) Product Image
    1/16
  • Experimental Validation Results for ARL13B Antibody (YA6956)
  • ARL13B Antibody (YA6956) Product Image
  • Experimental Validation Results for ARL13B Antibody (YA6956)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HL-60 (lane 2(20μg)) 、HepG2 (lane 3(20μg)) and K562 (lane 4(20μg)) using ARL13B Antibody (HY-P87273) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ARL13B Antibody (YA6956)
  • ARL13B Antibody (YA6956) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HL-60 (lane 2(20μg)) 、HepG2 (lane 3(20μg)) and K562 (lane 4(20μg)) using ARL13B Antibody (HY-P87273) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ARL13B Antibody (YA6956) Product Image WB