Thioredoxin Reductase 1 Antibody (YA2315)
(Synonyms: TR; TR1; TXNR; TRXR1; GRIM-12)
Based on 1 Customer Validation
Thioredoxin Reductase 1 Antibody (YA2315) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thioredoxin Reductase 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:100-1:200 | 1:100-1:200 | 1:100-1:500 | 1:10-1:100 |
Product Details
Thioredoxin Reductase 1 Antibody (YA2315) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thioredoxin Reductase 1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 71 kDa
Entrez Gene: 7296 Human ; 50493 Mouse ;
SwissProt: Q16881 Human ; Q9JMH6 Mouse ; O89049 Rat
OMIM: 601112 Human
Recombinant protein of human TXNRD1
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human liver cancer using Thioredoxin Reductase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82570, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using Thioredoxin Reductase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82570, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lymph node using Thioredoxin Reductase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82570, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Thioredoxin Reductase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82570, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human striated muscle using Thioredoxin Reductase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82570, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta using Thioredoxin Reductase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82570, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
Thioredoxin Reductase 1 reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2); Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions; Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2; Enhances the transcriptional activity of the estrogen receptor ESR2 only. Mediates cell death induced by a combination of interferon-beta and retinoic acid[1][2][3][4].
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Subcellular Localization
Cytoplasm; Cytoplasm; Nucleus; Cytoplasm
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Expression
Tissue_specificity:Expressed predominantly in Leydig cells (at protein level) . Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines; Widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver
Induction:Induced by estradiol or testosterone in HeLa cells; Induced by a combination of interferon-beta and retinoic acid (at protein level) -
Isoforms & Post-Translational Modification
Q16881 has 7 isomers: Q16881-1: 70906 Da (predicted); Q16881-2: 59787 Da (predicted); Q16881-3: 65567 Da (predicted); Q16881-4: 60419 Da (predicted); Q16881-5: 54754 Da (predicted); Q16881-6: 67266 Da (predicted); Q16881-7: 50873 Da (predicted).
The N-terminus is blocked;ISGylated -
Subunit
Homodimer (PubMed:17512005, PubMed:8577704). Interacts with HERC5
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SwissProt ID
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Synonyms
TR; TR1; TXNR; TRXR1; GRIM-12
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Research Field
Signal Transduction
Documentation
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