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4 Hydroxynonenal Antibody

(Synonyms: 4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; HNE; 4HNE; 4-HNE; (E)-4-Hydroxy-2-nonenal; 2-Nonenal, 4-hydroxy-, (2E)-; (2E)-4-Hydroxy-2-nonenal; 4-Hydroxy-2(E)-nonenal)
18 Cited Publications
Customer Review

Based on 18 publication(s) in Google Scholar

4 Hydroxynonenal Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 4 Hydroxynonenal.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P mIHC
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • 4 Hydroxynonenal Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) 4 Hydroxynonenal Antibody

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18 Publications Citing Use of MCE 4 Hydroxynonenal Antibody (6)
IHC
  • IHC
  • IHC
  • IHC
  • IHC
  • IHC
  • IHC

Applications

Application
WB Info
WB: Western Blot
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-2000 1:200-1000 1:200-1000 1:200-1000

Product Details

Description

4 Hydroxynonenal Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 4 Hydroxynonenal.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Species independent
  • Calculated Molecular Weight Predicted band size: 0.156 kDa
Immunogen

KLH conjugated 4-Hydroxynonenal.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103101

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded thyroid cancer tissue using 4 Hydroxynonenal Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81208, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    4 Hydroxynonenal is a 4-Hydroxynonenal (HY-113466): 4-Hydroxynonenal (4-HNE) is an α,β unsaturated hydroxyalkenal and an oxidative/nitrosative stress biomarker. 4-Hydroxynonenal is a substrate and an inhibitor of acetaldehyde dehydrogenase 2 (ALDH2) . 4-Hydroxynonenal can modulate a number of signaling processes mainly through forming covalent adducts with nucleophilic functional groups in proteins, nucleic acids, and membrane lipids. 4-Hydroxynonenal plays an important role in cancer through mitochondria.

  • Synonyms

    4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; HNE; 4HNE; 4-HNE; (E)-4-Hydroxy-2-nonenal; 2-Nonenal, 4-hydroxy-, (2E)-; (2E)-4-Hydroxy-2-nonenal; 4-Hydroxy-2(E)-nonenal

4 Hydroxynonenal Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded thyroid cancer tissue using 4 Hydroxynonenal Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81208, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Sep:85:103672. [Abstract]

    Customer Validation
     IHC staining of 4-HNE, and GPX4 in xenograft tumors across different treatment groups.

  • 1/16

    4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 13:e06497. [Abstract]

    Customer Validation
     4 Hydroxynonenal Antibody (diluted 1:400). Representative 4‐HNE‐stained images of ovarian tissues from the indicated groups of mice (scale bar, 20 µm).

  • 1/16

    4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 7;16(1):810. [Abstract]

    Customer Validation
     Representative images of H&E staining and IHC staining for SETD7, Ki67, ALDH1A3, and 4-HNE in subcutaneous xenograft tumors. Scale bar: 50 μm.

  • 1/16

    4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Sep 18:35:102317. [Abstract]

    Customer Validation
     4 Hydroxynonenal Antibody (1: 500). Representative immunohistochemical staining of 4-HNE and GPX4 in tumor sections from each treatment group (Ctrl, FGN, FGN-BBN, FGN+NIR, and FGN-BBN+NIR). Scale bar: 50 μm.

  • 1/16

    4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: J Future Foods. 2025 Oct 27.

    Customer Validation
     Representative immunohistochemical staining of 4 Hydroxynonenal (4-HNE) (brown).

  • 1/16

    4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 8:226:29-42. [Abstract]

    Customer Validation
     Representative IHC staining images showing levels of LRRC45 and 4-HNE (4 Hydroxynonenal) in bladder cancer tissues. The scale bars represent 200 μm (low magnification) and 50 μm (high magnification).

  • 4 Hydroxynonenal Antibody Product Image
    1/16
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • 4 Hydroxynonenal Antibody Product Image
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded thyroid cancer tissue using 4 Hydroxynonenal Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81208, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody
  • Experimental Validation Results for 4 Hydroxynonenal Antibody

  • 4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Sep:85:103672. [Abstract]

    IHC staining of 4-HNE, and GPX4 in xenograft tumors across different treatment groups.

  • 4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 13:e06497. [Abstract]

    4 Hydroxynonenal Antibody (diluted 1:400). Representative 4‐HNE‐stained images of ovarian tissues from the indicated groups of mice (scale bar, 20 µm).

  • 4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Nov 7;16(1):810. [Abstract]

    Representative images of H&E staining and IHC staining for SETD7, Ki67, ALDH1A3, and 4-HNE in subcutaneous xenograft tumors. Scale bar: 50 μm.

  • 4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Sep 18:35:102317. [Abstract]

    4 Hydroxynonenal Antibody (1: 500). Representative immunohistochemical staining of 4-HNE and GPX4 in tumor sections from each treatment group (Ctrl, FGN, FGN-BBN, FGN+NIR, and FGN-BBN+NIR). Scale bar: 50 μm.

  • 4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: J Future Foods. 2025 Oct 27.

    Representative immunohistochemical staining of 4 Hydroxynonenal (4-HNE) (brown).

  • 4 Hydroxynonenal Antibody purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Nov 8:226:29-42. [Abstract]

    Representative IHC staining images showing levels of LRRC45 and 4-HNE (4 Hydroxynonenal) in bladder cancer tissues. The scale bars represent 200 μm (low magnification) and 50 μm (high magnification).

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded thyroid cancer tissue using 4 Hydroxynonenal Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81208, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81208, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 4 Hydroxynonenal Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Esophageal Carcinoma tissue using 4 Hydroxynonenal antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81208, 1:800 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image IHC-P
  • 4 Hydroxynonenal Antibody Product Image mIHC
  • 4 Hydroxynonenal Antibody Product Image mIHC
  • 4 Hydroxynonenal Antibody Product Image mIHC
  • 4 Hydroxynonenal Antibody Product Image mIHC
  • 4 Hydroxynonenal Antibody Product Image mIHC
  • 4 Hydroxynonenal Antibody Product Image mIHC