8-OHdG (DNA/RNA Damage) Antibody

(Synonyms: 8-hydroxy-2'-deoxyguanosine; 2'-Deoxy-8-oxoguanosine; 7,8-Dihydro-8-oxo-2'-deoxyguanosine; 8-Oxo-7,8-dihydro-2'-deoxyguanosine; 8-Oxo-7,8-dihydrodeoxyguanosine; 2'-Deoxy-8-oxo-D-guanosine; 8-Oxo-7,8-dihydro-2μ-deoxyguanosine,8-Oxo-dG; 2'-Deoxy-8-hydroxyguanosine; )
18 Cited Publications
Customer Review

Based on 18 publication(s) in Google Scholar

8-OHdG (DNA/RNA Damage) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 8-OHdG (DNA/RNA Damage).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P, IHC-F, ELISA, IF-Tissue, mIHC

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated

Applications

Application
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:5000-10000 1:100-500 1:100-500 1:100-500 1:500

Product Details

Description

8-OHdG (DNA/RNA Damage) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 8-OHdG (DNA/RNA Damage).

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Species independent
  • Calculated Molecular Weight Predicted band size: 0.283kDa
Immunogen

KLH conjugated 8-OHdG

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103069

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for 8-OHdG (DNA/RNA Damage) Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 8-OHdG (DNA/RNA Damage) Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 8-OHdG (DNA/RNA Damage) Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 8-OHdG (DNA/RNA Damage) Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 8-OHdG (DNA/RNA Damage) Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for 8-OHdG (DNA/RNA Damage) Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)(HY-D1832). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    8-OHdG is a 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a marker formed in DNA after oxidation; guanine, the most sensitive base to free radical attack in DNA, can be converted to various markers such as 8-hydroxyguanine, 8-hydroxyguanosine, and 8-OHdG after oxidation. Among them, the 8-OHdG adduct is one of the most abundant base modifications excreted in urine, and due to its easy collection, it is commonly used as a biomarker for oxidative DNA damage. It is also the main product of DNA damage and can be used as an important biomarker to evaluate oxidative stress induced by exogenous compounds. Chronic arsenic exposure leads to elevated levels of urinary 8-OHdG, triclosan (TCS) exposure significantly increases 8-OHdG levels in zebrafish brain tissue, and levels of 8-OHdG in mitochondrial DNA isolated from the parietal cortex of patients with Alzheimer’s disease (AD) are significantly higher than those of age-matched controls. Additionally, the presence of 8-OHdG in DNA (also known as 8-hydroxyguanosine in RNA) is a reliable marker of base modifications, as it is consistently detected after oxidative stress generated by various conditions.

  • Synonyms

    8-hydroxy-2'-deoxyguanosine; 2'-Deoxy-8-oxoguanosine; 7,8-Dihydro-8-oxo-2'-deoxyguanosine; 8-Oxo-7,8-dihydro-2'-deoxyguanosine; 8-Oxo-7,8-dihydrodeoxyguanosine; 2'-Deoxy-8-oxo-D-guanosine; 8-Oxo-7,8-dihydro-2μ-deoxyguanosine,8-Oxo-dG; 2'-Deoxy-8-hydroxyguanosine;

8-OHdG (DNA/RNA Damage) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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