1. Metabolic Enzyme/Protease
    Autophagy
    Apoptosis
  2. Mitochondrial Metabolism
    Autophagy
    Apoptosis
  3. Rotenone

Rotenone 

Cat. No.: HY-B1756 Purity: 99.64%
Handling Instructions

Rotenone is an mitochondrial electron transport chain complex I inhibitor. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

For research use only. We do not sell to patients.

Rotenone Chemical Structure

Rotenone Chemical Structure

CAS No. : 83-79-4

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1  mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 108 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 10 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Rotenone purchased from MCE. Usage Cited in: Mediat Inflamm. 2020 Aug 17.

    Western blot analysis of NLRP3, IL-1β, IL-18, and caspase-1 p10 expression in renal tubular epithelial cells stimulated with Rotenone. Rotenone induces NLRP3 inflammasome activation.
    • Biological Activity

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    • References

    • Customer Review

    Description

    Rotenone is an mitochondrial electron transport chain complex I inhibitor. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

    In Vitro

    Mitogen Activated Protein Kinase (MAPK), Toll-like receptor, Wnt, and Ras signaling pathways are intensively involved in the effect of rotenone on the ENS[2]. Rotenone-induced cell death is reduced by MCE treatment as measured by decline in the levels of pro-apoptotic proteins. Moreover, MCE treatment significantly augments the levels of anti-apoptotic Bcl2 and blocks the release of cytochrome c, thereby alleviating the rotenone-induced dopaminergic neuronal loss, as evidenced by tyrosine hydroxylase (TH) immunostaining in the striatum[3].

    In Vivo

    Rotenone causes a significant increase in the excitatory amino acid neurotransmitters; glutamate and aspartate together with a significant decrease in the inhibitory amino acids, GABA, glycine and taurine are observed in the cerebellum of rat model of PD[1]. Rotenone (1.5, 2, or 2.5 mg/kg) causes a dose-dependent increase in α-synuclein in the substantia nigra. Furthermore, at 2 and 2.5 mg/kg, rotenone causes a significant decrease in the number of tyrosine hydroxylase-immunoreactive neurons in the substantia nigra, and dopamine in the striatum in rats[4].

    Molecular Weight

    394.42

    Formula

    C₂₃H₂₂O₆

    CAS No.

    83-79-4

    SMILES

    O=C1[[email protected]]2([H])[[email protected]](COC3=CC(OC)=C(OC)C=C32)([H])OC4=C5C(O[[email protected]@H](C(C)=C)C5)=CC=C14

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (126.77 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5354 mL 12.6768 mL 25.3537 mL
    5 mM 0.5071 mL 2.5354 mL 5.0707 mL
    10 mM 0.2535 mL 1.2677 mL 2.5354 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.64%

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    Keywords:

    RotenoneMitochondrial MetabolismAutophagyApoptosisInhibitorinhibitorinhibit

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    Product Name:
    Rotenone
    Cat. No.:
    HY-B1756
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