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  2. Cell-Derived Biogenetic Gold Nanoparticles for Sensitizing Radiotherapy and Boosting Immune Response against Cancer

Cell-Derived Biogenetic Gold Nanoparticles for Sensitizing Radiotherapy and Boosting Immune Response against Cancer

  • Small. 2021 Dec;17(50):e2103984. doi: 10.1002/smll.202103984.
Xianya Qin 1 Conglian Yang 1 Hongbo Xu 1 Runzan Zhang 1 Dan Zhang 2 Jingyao Tu 3 Yuanyuan Guo 4 Boning Niu 1 Li Kong 1 Zhiping Zhang 1 5 6
Affiliations

Affiliations

  • 1 Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 2 Department of Pharmacy, Wuhan First Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 3 Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 4 Liyuan Hospital, Huazhong University of Science and Technology, Wuhan, 430077, China.
  • 5 National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 6 Hubei Engineering Research Centre for Novel Drug Delivery System, Huazhong University of Science and Technology, Wuhan, 430030, China.
Abstract

The biosynthesis of nanomedicine has gained enormous attention and exhibited promising prospects, while the underlying mechanism and advantage remain not fully understood. Here, a cell-reactor based on tumor cells is developed to obtain biogenetic gold nanoparticles (Au@MC38) for sensitizing radiotherapy and boosting immune responses. It demonstrates that the intracellular biomineralization and exocytosis process of Au@MC38 can be regulated by the cellular metabolites level and other factors, such as glutathione and Reactive Oxygen Species (ROS), Autophagy, and UV irradiation. The elucidation of mechanisms may promote the understanding of interaction principles between nanoparticles and biosystems in the process of biosynthesis. Combined with radiotherapy, Au@MC38 strengthens the radiation-induced DNA damage and ROS generation, thus aggravating Cell Apoptosis and Necrosis. Benefiting from homologous targeting and transcytosis effect, Au@MC38 demonstrates good tumor distribution. Local radiation-induced immunogenic cell death initiates an effective immune response. Especially, CD8a+ dendritic cells are significantly increased in mice that received combinatorial treatment. This radio-sensitization strategy has demonstrated the effective inhibition on primary and metastatic tumors, and achieved satisfactory survival benefit in combinatorial with immune checkpoint blockade. Thus, this bio-inspired synthetic strategy may impulse the development of biosynthesis and its therapeutic applications, contributing to a non-invasive and efficient modality for nanomedicine exploitation.

Keywords

biosynthesis; exocytosis; gold nanoparticles; immunotherapy; radio-sensitization.

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