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  3. Tamoxifen

Tamoxifen  (Synonyms: ICI 47699; (Z)-Tamoxifen; trans-Tamoxifen)

Cat. No.: HY-13757A Purity: 99.88%
COA Handling Instructions

Tamoxifène (ICI 47699) est un modulateur sélectif des récepteurs des œstrogènes (SERM) qui bloque l'action des œstrogènes dans les cellules du sein et peut activer l'activité des œstrogènes dans d'autres cellules, comme les os, le foie, et les cellules utérines. Tamoxifène est un puissant activateur de Hsp90 et améliore l'activité d'ATPase du chaperon moléculaire Hsp90. Tamoxifène active l'autophagie et induit l'apoptosis.

Tamoxifen (ICI 47699) ist ein selektiver estrogen receptor-Modulator (SERM), der die Östrogenwirkung in Brustzellen blockiert und die Östrogenaktivität in anderen Zellen, wie Knochen-, Leber- und Gebärmutterzellen, aktivieren kann. Tamoxifen ist ein potenter Hsp90-Aktivator und steigert die Aktivität des molekularen Hsp90-Chaperons ATPase. Tamoxifen aktiviert die autophagy und induziert die apoptosis.

Tamoxifen (ICI 47699) is an orally active, selective estrogen receptor modulator (SERM) which blocks estrogen action in breast cells and can activate estrogen activity in other cells, such as bone, liver, and uterine cells. Tamoxifen is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen also potent inhibits infectious EBOV Zaire and Marburg (MARV) with IC50 of 0.1 µM and 1.8 µM, respectively. Tamoxifen activates autophagy and induces apoptosis. Tamoxifen also can induce gene knockout of CreER(T2) transgenic mouse.

For research use only. We do not sell to patients.

Tamoxifen Chemical Structure

Tamoxifen Chemical Structure

CAS No. : 10540-29-1

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Solid + Solvent
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 66 In-stock
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500 mg USD 60 In-stock
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5 g USD 288 In-stock
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Customer Review

Based on 83 publication(s) in Google Scholar

Other Forms of Tamoxifen:

Top Publications Citing Use of Products

80 Publications Citing Use of MCE Tamoxifen

WB

    Tamoxifen purchased from MedChemExpress. Usage Cited in: Redox Biol. 18 October 2022, 102509.

    Expression of TFRC in primary fibroblasts from TFRCflox/flox and TFRC−/− mice was assessed by western blot after 5 days of Tamoxifen intraperitoneal injection and 7 days of waiting.

    Tamoxifen purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2020 Jan;10(1):137-150.  [Abstract]

    Different transmission of MAPK8/FoxO signaling pathway in breast cancer cells (MCF-7, T47D, ZR-75, and MDA-MB-231) and liver cells (LO2) exposed to TAM (Tamoxifen).

    Tamoxifen purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2019 May 23;17(1):50.  [Abstract]

    Tamoxifen inhibits the growth and metastasis of prostate tumors in vivo. Western blot analysis of the indicated proteins in prostate primary tumors tissues.

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    Description

    Tamoxifen (ICI 47699) is an orally active, selective estrogen receptor modulator (SERM) which blocks estrogen action in breast cells and can activate estrogen activity in other cells, such as bone, liver, and uterine cells[1][2][3]. Tamoxifen is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen also potent inhibits infectious EBOV Zaire and Marburg (MARV) with IC50 of 0.1 µM and 1.8 µM, respectively[5]. Tamoxifen activates autophagy and induces apoptosis[4]. Tamoxifen also can induce gene knockout of CreER(T2) transgenic mouse[6].

    IC50 & Target[1][4]

    Estrogen receptor

     

    HSP90

     

    In Vitro

    Tamoxifen (ICI 47699) shows strong inhibition of MCF-7 cells (EC50=1.41 μM) and to a lesser extent the T47D cells (EC50=2.5 μM) but does not affect the MDA-MB-231 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Injection of pre-mutant mice with Tamoxifen (75 mg/kg; injected for 5 days at 6 weeks of age) results in the excision of the floxed exon and, thus, in a gene knockout[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Aldh1l1-cre/ERT2 x Ai95 mice[3]
    Dosage: 75 mg/kg
    Administration: Injected for 5 days at 6 weeks of age
    Result: Resulted in the excision of the floxed exon and a gene knockout.
    Clinical Trial
    Molecular Weight

    371.51

    Formula

    C26H29NO

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC/C(C1=CC=CC=C1)=C(C2=CC=CC=C2)/C3=CC=C(C=C3)OCCN(C)C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    Ethanol : 50 mg/mL (134.59 mM; Need ultrasonic)

    DMSO : 25 mg/mL (67.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6917 mL 13.4586 mL 26.9172 mL
    5 mM 0.5383 mL 2.6917 mL 5.3834 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  30% PEG400  0.5% Tween80  5% Propanediol  64.5%H2O

      Solubility: 5 mg/mL (13.46 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (6.73 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  Corn Oil

      Solubility: 40 mg/mL (107.67 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
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    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.92%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 2.6917 mL 13.4586 mL 26.9172 mL 67.2929 mL
    5 mM 0.5383 mL 2.6917 mL 5.3834 mL 13.4586 mL
    10 mM 0.2692 mL 1.3459 mL 2.6917 mL 6.7293 mL
    15 mM 0.1794 mL 0.8972 mL 1.7945 mL 4.4862 mL
    20 mM 0.1346 mL 0.6729 mL 1.3459 mL 3.3646 mL
    25 mM 0.1077 mL 0.5383 mL 1.0767 mL 2.6917 mL
    30 mM 0.0897 mL 0.4486 mL 0.8972 mL 2.2431 mL
    40 mM 0.0673 mL 0.3365 mL 0.6729 mL 1.6823 mL
    50 mM 0.0538 mL 0.2692 mL 0.5383 mL 1.3459 mL
    60 mM 0.0449 mL 0.2243 mL 0.4486 mL 1.1215 mL
    Ethanol 80 mM 0.0336 mL 0.1682 mL 0.3365 mL 0.8412 mL
    100 mM 0.0269 mL 0.1346 mL 0.2692 mL 0.6729 mL
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Tamoxifen
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