1. Metabolic Enzyme/Protease
  2. Mitochondrial Metabolism

Mito-TEMPO 

Cat. No.: HY-112879 Purity: >98.0%
Handling Instructions

Mito-TEMPO is a mitochondria-targeted superoxide dismutase mimetic with superoxide and alkyl radical scavenging properties.

For research use only. We do not sell to patients.

Mito-TEMPO Chemical Structure

Mito-TEMPO Chemical Structure

CAS No. : 1334850-99-5

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5 mg USD 180 Get quote

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Mito-TEMPO is a mitochondria-targeted superoxide dismutase mimetic with superoxide and alkyl radical scavenging properties.

In Vivo

Mito-TEMPO (MT) greatly attenuates the increase in ALT activities and reduces the areas of necrosis at both time points, indicating that the protection by Mito-TEMPO is sustained until at least 24 h post-APAP. Mito-Tempo could induce secondary apoptosis in the late phase of APAP hepatotoxicity. Mito-Tempo induces secondary apoptosis after APAP overdose by inhibition of RIP3[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9607 mL 9.8033 mL 19.6067 mL
5 mM 0.3921 mL 1.9607 mL 3.9213 mL
10 mM 0.1961 mL 0.9803 mL 1.9607 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[1]

Mice[1]
Male C57BL/6J mice (8-12 weeks) and RIP3-deficient mice (C57BL/6N background) are used throughout the study. The mice are acclimated before experiments with free access to diet and water. Overnight-fasted mice (16-18 h) are treated i.p. with 300 mg/kg APAP dissolved in warm saline. Some mice are treated with 200 mg/kg APAP in experiments evaluating effect of RIP3 deficiency. A dose of 20 mg/kg Mito-Tempo dissolved in saline is administered i.p. 1.5 or 3 h after APAP. Some mice are subsequently treated (i.p.) with 10 mg/kg ZVD fmk dissolved in Tris-buffered saline or vehicle 2 h after APAP. To mimic the clinical care of APAP-overdose patients, some mice receive the antidote NAC (i.p., 500 mg/kg) at 1.5 or 3 h after APAP overdose[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

510.03

Formula

C₂₉H₃₅ClN₂O₂P

CAS No.

1334850-99-5

SMILES

[O]N1C(C)(C)CC(NC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=O)CC1(C)C.[Cl-]

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

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Product Name:
Mito-TEMPO
Cat. No.:
HY-112879
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Mito-TEMPO

Cat. No.: HY-112879