1. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  2. Mitochondrial Metabolism Reactive Oxygen Species
  3. Mito-TEMPO

Mito-TEMPO 

Cat. No.: HY-112879 Purity: 98.35%
COA Handling Instructions

Mito-TEMPO is a mitochondria-targeted superoxide dismutase mimetic with superoxide and alkyl radical scavenging properties.

For research use only. We do not sell to patients.

Mito-TEMPO Chemical Structure

Mito-TEMPO Chemical Structure

CAS No. : 1334850-99-5

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
5 mg USD 65 In-stock
10 mg USD 110 In-stock
50 mg USD 420 In-stock
100 mg USD 700 In-stock
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Customer Review

Based on 52 publication(s) in Google Scholar

Other Forms of Mito-TEMPO:

Top Publications Citing Use of Products

49 Publications Citing Use of MCE Mito-TEMPO

WB
IF

    Mito-TEMPO purchased from MedChemExpress. Usage Cited in: Leukemia. 2023 Feb 4.  [Abstract]

    Mito-TEMPO (MTTP; 50 nM; 48 h) efficiently reduces total and mitochondrial ROS content in PDX AML cells. CellROX dye, left panel, MitoSOX dye, right panel.

    Mito-TEMPO purchased from MedChemExpress. Usage Cited in: Redox Biol. 2020 Jul;34:101559.  [Abstract]

    GS carbonylation in astrocytes under OGD/R. Mito-TEMPO decreases GS carbonylation in astrocytes.

    Mito-TEMPO purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 May 5;11(5):319.  [Abstract]

    Western blot assay shows the expression of nestin, PINK1, LC3 and p62 in the MPCs, which are pretreated with Mito-TEMPO and exposed to lupus nephritis plasma for 24 h.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Mito-TEMPO is a mitochondria-targeted superoxide dismutase mimetic with superoxide and alkyl radical scavenging properties[1].

    In Vivo

    Mito-TEMPO (MT) greatly attenuates the increase in ALT activities and reduces the areas of necrosis at both time points, indicating that the protection by Mito-TEMPO is sustained until at least 24 h post-APAP. Mito-Tempo could induce secondary apoptosis in the late phase of APAP hepatotoxicity. Mito-Tempo induces secondary apoptosis after APAP overdose by inhibition of RIP3[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    510.03

    Formula

    C29H35ClN2O2P

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to pink

    SMILES

    [O]N1C(C)(C)CC(NC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=O)CC1(C)C.[Cl-]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (245.08 mM; Need ultrasonic)

    H2O : 60 mg/mL (117.64 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9607 mL 9.8033 mL 19.6067 mL
    5 mM 0.3921 mL 1.9607 mL 3.9213 mL
    10 mM 0.1961 mL 0.9803 mL 1.9607 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (98.03 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.25 mg/mL (4.41 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.25 mg/mL (4.41 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.25 mg/mL (4.41 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.35%

    References
    Animal Administration
    [1]

    Mice[1]
    Male C57BL/6J mice (8-12 weeks) and RIP3-deficient mice (C57BL/6N background) are used throughout the study. The mice are acclimated before experiments with free access to diet and water. Overnight-fasted mice (16-18 h) are treated i.p. with 300 mg/kg APAP dissolved in warm saline. Some mice are treated with 200 mg/kg APAP in experiments evaluating effect of RIP3 deficiency. A dose of 20 mg/kg Mito-Tempo dissolved in saline is administered i.p. 1.5 or 3 h after APAP. Some mice are subsequently treated (i.p.) with 10 mg/kg ZVD fmk dissolved in Tris-buffered saline or vehicle 2 h after APAP. To mimic the clinical care of APAP-overdose patients, some mice receive the antidote NAC (i.p., 500 mg/kg) at 1.5 or 3 h after APAP overdose[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    Product Name:
    Mito-TEMPO
    Cat. No.:
    HY-112879
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