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STK33 Antibody (YA2085)(PBS only)

(Synonyms: STK33; Serine/threonine-protein kinase 33)

STK33 Antibody (YA2085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STK33.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for STK33 Antibody (YA2085)(PBS only)
  • STK33 Antibody (YA2085)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:100-1:200 1:500-1:1000

Product Details

Description

STK33 Antibody (YA2085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STK33.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Immunogen

Recombinant protein of human STK33

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STK33 Antibody (YA2085)(PBS only)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293 (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), and A431 (lane 4, 15 μg) using STK33 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Serine/Threonine Kinase 33 (STK33) plays a pivotal role in cancer cell proliferation and metastasis by phosphorylating serine and threonine residues on proteins, thereby regulating crucial cellular processes such as signal transduction, DNA replication, cell differentiation, proliferation, apoptosis, and tumor development. STK33 is overexpressed in various cancers, including pancreatic, lung, and liver cancers, compared to non-cancerous tissues. Notably, STK33 activates the PI3K/Akt/mTOR signaling pathway in gastric cancer, promoting metastasis. Additionally, the STK33/ERK2 signaling pathway contributes to the tumorigenesis of colorectal cancer by phosphorylating ERK2, enhancing its activity and promoting cancer progression. STK33's involvement in cancer development underscores its importance in oncology research.[1][2].

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system

  • Isoforms & Post-Translational Modification

    Q9BYT3 has 2 isomers: Q9BYT3-1: 57831 Da (predicted); Q9BYT3-2: 50176 Da (predicted).
    Autophosphorylated

  • Subunit

    Homodimer

  • SwissProt ID

    Q9BYT3

  • Gene ID
    65975 [NCBI]
  • Synonyms

    STK33; Serine/threonine-protein kinase 33

  • Research Field

    Signal Transduction

References

[1]. Jiang H, et al. Serine/Threonine Kinase (STK) 33 promotes the proliferation and metastasis of human esophageal squamous cell carcinoma via inflammation-related pathway. Pathol Res Pract. 2024 Feb;254:155154. [Content Brief]

[2]. Zhang S, et al. STK33/ERK2 signal pathway contribute the tumorigenesis of colorectal cancer HCT15 cells. Biosci Rep. 2019 Mar 1;39(3):BSR20182351. [Content Brief]

STK33 Antibody (YA2085)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293 (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), and A431 (lane 4, 15 μg) using STK33 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • STK33 Antibody (YA2085)(PBS only) Product Image
    1/16
  • Experimental Validation Results for STK33 Antibody (YA2085)(PBS only)
  • STK33 Antibody (YA2085)(PBS only) Product Image
  • Experimental Validation Results for STK33 Antibody (YA2085)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293 (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), and A431 (lane 4, 15 μg) using STK33 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for STK33 Antibody (YA2085)(PBS only)
  • STK33 Antibody (YA2085)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), 293 (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), and A431 (lane 4, 15 μg) using STK33 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • STK33 Antibody (YA2085)(PBS only) Product Image WB