VEGFR3 Antibody (YA4861)
(Synonyms: FLT4; VEGFR3; Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4)VEGFR3 Antibody (YA4861) is a Rabbit-derived and non-conjugated recombinant monoclonal antibody, targeting to VEGFR3.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin 950.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:2000-1:5000 | 1:200-1000 |
Product Details
VEGFR3 Antibody (YA4861) is a Rabbit-derived and non-conjugated recombinant monoclonal antibody, targeting to VEGFR3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 153 kDa;
Recombinant Human VEGFR3 protein
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin 950.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
VEGFR3 is a Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in human lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cytoplasm; Nucleus; Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted; Cytoplasm
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Expression
Tissue_specificity:Detected in endothelial cells (at protein level) . Widely expressed. Detected in fetal spleen, lung and brain. Detected in human liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney -
Isoforms & Post-Translational Modification
P35916 has 3 isomers: P35916-2: 152757 Da (predicted); P35916-1: 145599 Da (predicted); P35916-3: 93174 Da (predicted).
Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation in response to H(2)O(2) is mediated by a process that requires SRC and PRKCD activity. Phosphorylation at Tyr-1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at Tyr-1063 and Tyr-1337 is important for interaction with CRK and subsequent activation of MAPK8. Phosphorylation at Tyr-1230, Tyr-1231 and Tyr-1337 is important for interaction with GRB2 and subsequent activation of the AKT1 and MAPK1/ERK2 and/or MAPK3/ERK1 signaling pathways. In response to endothelial cell adhesion onto collagen, can also be phosphorylated in the absence of FLT4 kinase activity by SRC at Tyr-830, Tyr-833, Tyr-853, Tyr-1063, Tyr-1333, and Tyr-1337 -
Subunit
Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR.
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SwissProt ID
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Synonyms
FLT4; VEGFR3; Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4
Documentation
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