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TGF alpha Antibody (YA037)

(Synonyms: ETGF; TFGA; TGF A; TGF-alpha; Tgfa; Wa1; Waved 1)
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Based on 1 Customer Validation

TGF alpha Antibody (YA037) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TGF alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TGF alpha Antibody (YA037)
  • TGF alpha Antibody (YA037) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

TGF alpha Antibody (YA037) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TGF alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Species Reactivity Database

Entrez Gene: 7039 Human

SwissProt: P01135 Human

Immunogen

Synthetic peptide corresponding to Human TGF alpha.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102251

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TGF alpha Antibody (YA037)
    Western blot analysis of extracts from K562(20μg) using TGF alpha (HY-P80911) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80911, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Transforming growth factor α (TGF-alpha; TGF-α) is a mitogenic polypeptide member of the epidermal growth factor (EGF) family of cytokines. TGF-α is composed of 50 amino acids, and displays 35-40% homology with EGF which is the prototypic member of the EG

  • Subcellular Localization

    Secreted, extracellular space; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines

  • Isoforms & Post-Translational Modification

    P01135 has 5 isomers: P01135-1: 17006 Da (predicted); P01135-2: 16935 Da (predicted); P01135-3: 17079 Da (predicted); P01135-4: 17400 Da (predicted); P01135-5: 17329 Da (predicted).

  • Subunit

    Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2

  • SwissProt ID

    P01135

  • Gene ID
    7039 [NCBI]
  • Synonyms

    ETGF; TFGA; TGF A; TGF-alpha; Tgfa; Wa1; Waved 1

  • Research Field

    Cardiovascular

TGF alpha Antibody (YA037) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(20μg) using TGF alpha (HY-P80911) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80911, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TGF alpha Antibody (YA037) Product Image
    1/16
  • Experimental Validation Results for TGF alpha Antibody (YA037)
  • TGF alpha Antibody (YA037) Product Image
  • Experimental Validation Results for TGF alpha Antibody (YA037)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(20μg) using TGF alpha (HY-P80911) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80911, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TGF alpha Antibody (YA037)
  • TGF alpha Antibody (YA037) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(20μg) using TGF alpha (HY-P80911) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80911, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TGF alpha Antibody (YA037) Product Image WB