Copy Link Print Information

Annexin V Antibody (YA1829)(PBS only)

(Synonyms: PP4; ANX5; ENX2; RPRGL3.)

Annexin V Antibody (YA1829) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin V.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
  • Annexin V Antibody (YA1829)(PBS only) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Annexin V Antibody (YA1829) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin V.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Immunogen

A synthetic peptide of human Annexin V

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
    Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Annexin V is a This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade

  • Subunit

    Monomer. Binds ATRX and EIF5B (By similarity). Interacts with hepatitis B virus (HBV)

  • SwissProt ID

    P08758

  • Gene ID
    308 [NCBI]
  • Synonyms

    PP4; ANX5; ENX2; RPRGL3.

  • Research Field

    Cell Biology

Annexin V Antibody (YA1829)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Annexin V Antibody (YA1829)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
  • Annexin V Antibody (YA1829)(PBS only) Product Image
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
  • Experimental Validation Results for Annexin V Antibody (YA1829)(PBS only)
  • Annexin V Antibody (YA1829)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Annexin V Antibody (YA1829)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Annexin V Antibody (YA1829)(PBS only) Product Image WB
  • Annexin V Antibody (YA1829)(PBS only) Product Image WB