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Annexin V Antibody (YA1829)

(Synonyms: PP4; ANX5; ENX2; RPRGL3.)
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Based on 1 Customer Validation

Annexin V Antibody (YA1829) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin V.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Annexin V Antibody (YA1829)
  • Experimental Validation Results for Annexin V Antibody (YA1829)
  • Annexin V Antibody (YA1829) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Annexin V Antibody (YA1829) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin V.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Annexin V

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104202

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Annexin V Antibody (YA1829)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Annexin V Antibody (YA1829)
    Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Annexin V is a This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade

  • Subunit

    Monomer. Binds ATRX and EIF5B (By similarity). Interacts with hepatitis B virus (HBV)

  • SwissProt ID

    P08758

  • Gene ID
    308 [NCBI]
  • Synonyms

    PP4; ANX5; ENX2; RPRGL3.

  • Research Field

    Cell Biology

Annexin V Antibody (YA1829) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Annexin V Antibody (YA1829) Product Image
    1/16
  • Experimental Validation Results for Annexin V Antibody (YA1829)
  • Experimental Validation Results for Annexin V Antibody (YA1829)
  • Annexin V Antibody (YA1829) Product Image
  • Experimental Validation Results for Annexin V Antibody (YA1829)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Annexin V Antibody (YA1829)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Annexin V Antibody (YA1829)
  • Experimental Validation Results for Annexin V Antibody (YA1829)
  • Annexin V Antibody (YA1829) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 3T3 (lane 2, 15 μg), K562 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using Annexin V Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Annexin V Antibody (YA1829) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg), NIH3T3 (lane 4(20μg) using Annexin V (HY-P82084) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Annexin V Antibody (YA1829) Product Image WB
  • Annexin V Antibody (YA1829) Product Image WB