Nesprin3 Antibody (YA1371)(PBS only)

Cat. No.: HY-P81626A: Data Sheet User Guide for Antibodies
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Nesprin3 Antibody (YA1371) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin3.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Nesprin3 Antibody (YA1371) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin3.

Host

Rabbit

분자량

Predicted band size: 112 kDa;

Observed band size: 112 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6ZMZ3

Gene ID

161176 [NCBI]

Immunogen

Recombinant protein of human Nesprin3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells

Subcellular Localization：Nucleus outer membrane; Single-pass type IV membrane protein; Nucleus envelope; Rough endoplasmic reticulum

Expression：
Tissue_specificity:Expressed in aortic endothelial cells (at protein level)

Isoforms & Post-Translational Modification：Q6ZMZ3 has 3 isomers: Q6ZMZ3-1: 112216 Da (predicted); Q6ZMZ3-2: 111677 Da (predicted); Q6ZMZ3-3: 68582 Da (predicted).
The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces

Subunit：Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. Interacts with SUN1 and SUN2; probably forming respective LINC complexes. Interacts with PLEC (via actin-binding domain). Interacts with DST. Interacts with SYNE1 via spectrin repeats. Interacts (via KASH domain) with TOR1A (ATP-bound); the interaction is required for SYNE3 nuclear envelope localization

Research Field

Signal Transduction

Synonyms

KASH3; NET53; Nesp3; C14orf49; C14orf139; LINC00341; NCRNA00341

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/ovcar-3-cell.html [Content Brief]