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PHGDH Antibody (YA6971)

(Synonyms: 3 PGDH antibody; 3-PGDH antibody; 3-phosphoglycerate dehydrogenase antibody; 3PGDH antibody; D-3-phosphoglycerate dehydrogenase antibody; EC 1.1.1.95 antibody; Epididymis secretory protein Li 113 antibody; HEL S 113 antibody; NLS antibody; NLS1 antibody)
Customer Review

Based on 1 Customer Validation

PHGDH Antibody (YA6971) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PHGDH.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PHGDH Antibody (YA6971)
  • Experimental Validation Results for PHGDH Antibody (YA6971)
  • PHGDH Antibody (YA6971) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:100 1:1000

Product Details

Description

PHGDH Antibody (YA6971) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PHGDH.

  • Host Mouse
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Immunogen

Recombinant protein within human PHGDH aa 1-533.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PHGDH Antibody (YA6971)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) and NIH-3T3 (lane 2(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHGDH Antibody (YA6971)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PHGDH catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate

  • Expression

    Induction: Induced by 17-beta-estradiol (estrogenic ligand) and 4-hydroxytamoxifen (agonist/antagonist ligand). Positively regulated by the transcription factors SP1 and NF-Y

  • Isoforms & Post-Translational Modification

    O43175: 533 amino acids, molecular weight 56651 Da.

  • Subunit

    Homotetramer

  • SwissProt ID

    O43175

  • Synonyms

    3 PGDH antibody; 3-PGDH antibody; 3-phosphoglycerate dehydrogenase antibody; 3PGDH antibody; D-3-phosphoglycerate dehydrogenase antibody; EC 1.1.1.95 antibody; Epididymis secretory protein Li 113 antibody; HEL S 113 antibody; NLS antibody; NLS1 antibody

PHGDH Antibody (YA6971) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) and NIH-3T3 (lane 2(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PHGDH Antibody (YA6971) Product Image
    1/16
  • Experimental Validation Results for PHGDH Antibody (YA6971)
  • Experimental Validation Results for PHGDH Antibody (YA6971)
  • PHGDH Antibody (YA6971) Product Image
  • Experimental Validation Results for PHGDH Antibody (YA6971)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) and NIH-3T3 (lane 2(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHGDH Antibody (YA6971)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHGDH Antibody (YA6971)
  • Experimental Validation Results for PHGDH Antibody (YA6971)
  • PHGDH Antibody (YA6971) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) and NIH-3T3 (lane 2(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PHGDH Antibody (YA6971) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) using PHGDH Antibody (HY-P87288) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PHGDH Antibody (YA6971) Product Image WB
  • PHGDH Antibody (YA6971) Product Image WB