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Cat. No.: HY-P89420

HPV16 E7 Antibody

Name: HPV16 E7 Antibody
Brand: MedChemExpress
SKU: HY-P89420
Price: 130.00 USD
Availability: InStock
Rating: 5 (1 reviews)

Customer Review

Based on 1 Customer Validation

HPV16 E7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to HPV16 E7.

For research use only. We do not sell to patients.

Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Size

Price

Stock

Quantity

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	IHC-P IHC-P: Immunohistochemistry-Paraffin	IHC-F IHC-F: Immunohistochemistry-Frozen	IF-Tissue IF-Tissue: Immunofluorescence-Tissue	FC FC: Flow Cytometry
Dilution Ratio	1:500-1000	1:100-500	1:100-500	1:100-500	1ug/Test

Description

HPV16 E7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to HPV16 E7.

Host Rabbit
Clonality Polyclonal
Species Reactivity
Virus
Observed Molecular Weight

Observed band size: 18 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 11 kDa

Immunogen

KLH conjugated synthetic peptide derived from human HPV16 E7 : 21-98/98

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Appearance

Solution
Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Shipping

Shipping with blue ice.

Function

HPV16 E7 is a multifunctional nuclear phosphoprotein and a key oncoprotein in HPV16 carcinogenesis. It belongs to the human papillomavirus early protein family. The C-terminal half of HPV16 E7 contains two conserved Cys-X-X-Cys motifs, which can reversibly bind Zn2+ and Cd2+, significantly increase the α-helical content, and stabilize the hydrophobic core of the protein. The amino terminus of HPV16 E7 has sequence similarity with the adenovirus E1A protein and functional commonality with the SV40 large T antigen. HPV16 E7 binds to the retinoblastoma protein (pRB) with high affinity, promotes its degradation through the proteasome pathway, and releases the transcription factor E2F to activate cell cycle progression. HPV16 E7 cooperates with the Ras oncogene to transform primary rodent cells. HPV16 E7 synergizes with E6 to immortalize primary human keratinocytes and neutralizes the cytotoxic effects of E5. HPV16 E7 downregulates E-cadherin expression and enhances cell migration and invasion by activating AP-1 and NF-κB pathways. HPV16 E7 also disrupts cell adhesion and promotes epithelial-mesenchymal transition by interfering with cell-cell junctions and cytoskeletal rearrangement. Upstream regulatory factors of HPV16 E7 include viral promoters (such as LCR); downstream targets include pRB, E2F, p16 and E-cadherin, which can affect cell cycle control and adhesion molecule expression.