Phospho-SHIP1 (Tyr1020) Antibody (YA3089)

Cat. No.: HY-P83344: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-SHIP1 (Tyr1020) Antibody (YA3089) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-SHIP1 (Tyr1020).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-SHIP1 (Tyr1020) Antibody (YA3089) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-SHIP1 (Tyr1020) Antibody (YA3089) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-SHIP1 (Tyr1020).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 133 kDa;

Observed band size: 145 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q92835

Gene ID

3635 [NCBI]

Immunogen

A synthesized peptide derived from human SHIP PL-pY-GS.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed:10764818, PubMed:8723348, PubMed:8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:10764818, PubMed:8769125, PubMed:9108392). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed:16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression

Subcellular Localization：Cytoplasm; Cell membrane; Peripheral membrane protein; Membrane raft; Cytoplasm, cytoskeleton; Membrane; Peripheral membrane protein

Expression：
Tissue_specificity:This protein is specifically expressed on immune cells and hematopoietic cells. It is expressed in both bone marrow and blood cells. Its expression level varies significantly across different cell populations. It is expressed in at least 74% of immature CD34+ cells, while only 10% of more mature CD33+ cells express it. This protein is expressed in most T cells, and in a minority of B cells (protein level) .

Isoforms & Post-Translational Modification：Q92835 has 3 isomers: Q92835-1: 133292 Da (predicted); Q92835-2: 133193 Da (predicted); Q92835-3: 109294 Da (predicted).
Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding

Subunit：Interacts with tyrosine phosphorylated form of SHC1 (PubMed:8874179). Interacts with tyrosine phosphorylated form of DOK1 (PubMed:10822173)

Database

Entrez Gene: 3635 Human

SwissProt: Q92835 Human

OMIM: 601582 Human

Research Field

Immunology

Synonyms

INPP5D; SHIP; SHIP1; Phosphatidylinositol 3; 4; 5-trisphosphate 5-phosphatase 1; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-SHIP1 (Tyr1020) Antibody (YA3089) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-SHIP1 (Tyr1020) Antibody (YA3089)

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Product Detail

Background

Documentation

Phospho-SHIP1 (Tyr1020) Antibody (YA3089) Related Classifications

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