Cytokeratin 18 Antibody (YA3661)(PBS only)

Cat. No.: HY-P83964A: Data Sheet User Guide for Antibodies
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Cytokeratin 18 Antibody (YA3661) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 18.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cytokeratin 18 Antibody (YA3661) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 18.

Host

Mouse

Molecular Weight

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P05783

Gene ID

3875 [NCBI]

Immunogen

Purified recombinant fragment of human KRT18 (AA: 1-150) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection

Subcellular Localization：Nucleus matrix; Cytoplasm, perinuclear region; Nucleus, nucleolus; Cytoplasm

Expression：
Tissue_specificity:It is expressed in the colon, placenta, and liver, with very weak expression at the external os of the cervix. Increased expression has been observed in breast cancer lymph nodes.

Induction:By IL6/interleukin-6

Subunit：Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8 (PubMed:10852826, PubMed:24940650). Interacts with PLEC isoform 1C, when in a heterodimer with KRT8 (PubMed:24940650).

Synonyms

K18; CK-18; CYK18

Documentation

User Guide for Antibodies (1077 KB)