NFAT2 Antibody (YA270)

Cat. No.: HY-P80243: Data Sheet COA
SDS
User Guide for Antibodies
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NFAT2 Antibody (YA270) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NFAT2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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NFAT2 Antibody (YA270) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

NFAT2 Antibody (YA270) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NFAT2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 101 kDa;

Observed band size: 101 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95644

Gene ID

4772 [NCBI]

Immunogen

Synthetic peptide corresponding to Human NFAT2.AA range:894-943.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB FC

Western blot analysis of extracts from K562 (lane 2(20μg), K562 (lane 3(40μg), using NFAT2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 K562 cells labeling NFAT2 Antibody (HY-P80243, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed:10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:This protein is expressed in the thymus, peripheral blood leukocytes (such as T cells) , and spleen. The A subtype is preferentially expressed in effector T cells (thymus and peripheral blood leukocytes) , while the B and C subtypes are preferentially expressed in naive T cells (spleen) . The B subtype is expressed after the first exposure of naive T cells to the antigen, and the A subtype is expressed after the second exposure of effector T cells to the antigen. The IA subtype is widely expressed but not detected in the liver and pancreas; its neural expression is strongest in the corpus callosum. The IB subtype is mainly expressed in muscle, cerebellum, placenta, and thymus; neural expression is present in all of these areas of the human brain, with the strongest expression in the corpus callosum.

Induction:Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A

Isoforms & Post-Translational Modification：O95644 has 10 isomers: O95644-1: 101243 Da (predicted); O95644-2: 77785 Da (predicted); O95644-3: 76879 Da (predicted); O95644-4: 88763 Da (predicted); O95644-5: 87857 Da (predicted); O95644-6: 100336 Da (predicted); O95644-8: 74276 Da (predicted); O95644-10: 77376 Da (predicted); O95644-11: 76469 Da (predicted); O95644-17: 38626 Da (predicted).
Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1

Subunit：Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers (PubMed:9506523). Interacts with HOMER2 and HOMER3; this interaction may compete with calcineurin/PPP3CA-binding and hence prevent NFATC1 dephosphorylation and activation (PubMed:18218901). Interacts with TLE6/GRG6 (By similarity)

RRID

AB_3102374

Database

Entrez Gene: 4772 Human

SwissProt: O95644 Human

OMIM: 600489 Human

Research Field

Immunology

Synonyms

cytoplasmic 1 antibody; MGC138448 antibody; NF ATc antibody; NF ATc1 antibody; NF-ATc antibody; NF-ATc1 antibody; NF-ATc1.2 antibody; NFAC1_HUMAN antibody; NFAT 2 antibody; NFAT transcription complex cytosolic component antibody; NFATC 1 antibody; NFATc antibody; NFATc1 antibody; Nuclear factor of activated T cells cytoplasmic 1 antibody; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody; Nuclear factor of activated T cells cytosolic component 1 antibody; nuclear factor of activated T-cells 'c' antibody; Nuclear factor of activated T-cells antibody

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

NFAT2 Antibody (YA270) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NFAT2 Antibody (YA270)

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