Mast cell tryptase(PT2025) Antibody (YA5503)

製品番号: HY-P85811: データシート SDS COA User Guide for Antibodies
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Mast cell tryptase(PT2025) Antibody (YA5503) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Mast cell tryptase(PT2025).

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容量	価格（税別）	在庫状況	数量
20 μL	$115	在庫あり	Estimated Time of Arrival: December 31
50 μL	$185	在庫あり	Estimated Time of Arrival: December 31
100 μL	$300	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Mast cell tryptase(PT2025) Antibody (YA5503) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Mast cell tryptase(PT2025).

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 30 kDa;

Observed band size: 30 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15661

Gene ID

7177 [NCBI]

Immunogen

Synthesized peptide derived from human Mast cell tryptase AA range: 200-275

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

純度	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431 (lane2(20μg), K562 (lane3(20μg), Mouse liver tissue (lane4(20μg) and Rat liver tissue (lane5(20μg) using Mast cell tryptase(PT2025) Antibody (HY-P85811). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Isomer 1 and isomer 2 are expressed in the lungs, stomach, spleen, heart, and skin; in these tissues, isomer 1 is dominant. Isomer 2 is expressed in aortic, splenic, and breast tumors, with the highest expression levels in endothelial cells of certain vessels around the aorta and in endothelial cells around tumors, while expression levels are very low (at the protein level) in mast cells.

Isoforms & Post-Translational Modification：Q15661 has 2 isomers: Q15661-1: 30515 Da (predicted); Q15661-2: 29533 Da (predicted).

Subunit：Homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors, e.g. fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)