Phospho-Filamin A/B (Ser2152/Ser2107) Antibody (YA2590)(PBS only)

Cat. No.: HY-P82845A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Phospho-Filamin A/B (Ser2152/Ser2107) Antibody (YA2590) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Filamin A/B (Ser2152/Ser2107).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-Filamin A/B (Ser2152/Ser2107) Antibody (YA2590) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Filamin A/B (Ser2152/Ser2107).

Host

Rabbit

Molecular Weight

Predicted band size: 278 kDa;

Observed band size: 281 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

O75369

Gene ID

2317 [NCBI]

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser2152 of human Filamin A

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro

Subcellular Localization：Cytoplasm, cell cortex; Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, myofibril, sarcomere, Z line; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC) , retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT) , senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD) . Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart

Isoforms & Post-Translational Modification：O75369 has 9 isomers: O75369-1: 278164 Da (predicted); O75369-2: 275668 Da (predicted); O75369-3: 273909 Da (predicted); O75369-7: 257551 Da (predicted); O75369-4: 230788 Da (predicted); O75369-5: 230289 Da (predicted); O75369-6: 271413 Da (predicted); O75369-8: 281635 Da (predicted); O75369-9: 276939 Da (predicted).
ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling;Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation which promotes muscle differentiation

Subunit：Homodimer. Interacts with MICALL2 (By similarity). Interacts with RFLNA and RFLNB (By similarity). Isoform 1 interacts with FBLP1, FLNA, FLNC, GP1BA, INPPL1, ITGB1A, PSEN1 and PSEN2. Isoform 3 interacts with ITGB1A, ITGB1D, ITGB3 and ITGB6. Interacts with MYOT and MYOZ1. Interacts with HBV capsid protein. Interacts with ASB2 isoform 1; the interaction targets FLNB for proteasomal degradation (By similarity)

Research Field

Synonyms

FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280

Documentation

User Guide for Antibodies (1077 KB)