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PI3 Kinase p55 gamma Antibody

(Synonyms: GRB1; P85A; PI3-kinase p85-alpha subunit; PI3K; PI3K p85-alpha; P55G; PI3-kinase p85-gamma subunit; PI3K p85-gamma)
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Based on 1 publication(s) in Google Scholar

PI3 Kinase p55 gamma Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PI3 Kinase p55 gamma.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • PI3 Kinase p55 gamma Antibody Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

PI3 Kinase p55 gamma Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PI3 Kinase p55 gamma.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human PI3 Kinase p55 gamma aa412-461.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102236

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
    Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PI3 Kinase p55 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
    Immunocytochemistry analysis of SH-SY5Y cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
    Immunocytochemistry analysis of PC-3 cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PI3 Kinase p55 gamma binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1

  • Expression


    Tissue_specificity:It is found in the highest concentrations in the brain and testes. Lower concentrations are found in adipose tissue, kidneys, heart, lungs, and skeletal muscle.

  • Isoforms & Post-Translational Modification

    Q92569 has 3 isomers: Q92569-1: 54448 Da (predicted); Q92569-2: 47348 Da (predicted); Q92569-3: 43465 Da (predicted).

  • Subunit

    Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL

  • SwissProt ID

    Q92569

  • Gene ID
    8503 [NCBI]
  • Synonyms

    GRB1; P85A; PI3-kinase p85-alpha subunit; PI3K; PI3K p85-alpha; P55G; PI3-kinase p85-gamma subunit; PI3K p85-gamma

  • Research Field

    Signal Transduction

PI3 Kinase p55 gamma Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PI3 Kinase p55 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PI3 Kinase p55 gamma Antibody Product Image
    1/16
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • PI3 Kinase p55 gamma Antibody Product Image
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PI3 Kinase p55 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • Experimental Validation Results for PI3 Kinase p55 gamma Antibody
  • PI3 Kinase p55 gamma Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PI3 Kinase p55 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PI3 Kinase p55 gamma Antibody Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PI3 Kinase p55 gamma Antibody Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PI3 Kinase p55 gamma Antibody Product Image WB
  • PI3 Kinase p55 gamma Antibody Product Image ICC
  • PI3 Kinase p55 gamma Antibody Product Image ICC