PI3 Kinase p55 gamma Antibody

Cat. No.: HY-P80866: Data Sheet COA
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User Guide for Antibodies
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PI3 Kinase p55 gamma Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PI3 Kinase p55 gamma.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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PI3 Kinase p55 gamma Antibody Featured Recommendations:

1 Publications Citing Use of MCE PI3 Kinase p55 gamma Antibody

•SSRN. 2025 Sep 23.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

PI3 Kinase p55 gamma Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PI3 Kinase p55 gamma.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q92569

Gene ID

8503 [NCBI]

Immunogen

Synthetic peptide corresponding to Human PI3 Kinase p55 gamma aa412-461.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PI3 Kinase p55 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of SH-SY5Y cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of PC-3 cells labeling PI3 Kinase p55 gamma with PI3 Kinase p55 gamma Antibody (HY-P80866) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PI3 Kinase p55 gamma Antibody ((HY-P80866) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1

Expression：
Tissue_specificity:It is found in the highest concentrations in the brain and testes. Lower concentrations are found in adipose tissue, kidneys, heart, lungs, and skeletal muscle.

Isoforms & Post-Translational Modification：Q92569 has 3 isomers: Q92569-1: 54448 Da (predicted); Q92569-2: 47348 Da (predicted); Q92569-3: 43465 Da (predicted).

Subunit：Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL

RRID

AB_3102236

Database

Entrez Gene: 8503 Human ; 18710 Mouse ; 60664 Rat

SwissProt: Q92569 Human ; Q64143 Mouse ; Q63789 Rat

OMIM: 606076 Human

Research Field

Signal Transduction

Synonyms

GRB1; P85A; PI3-kinase p85-alpha subunit; PI3K; PI3K p85-alpha; P55G; PI3-kinase p85-gamma subunit; PI3K p85-gamma

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PI3 Kinase p55 gamma Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PI3 Kinase p55 gamma Antibody

PI3 Kinase p55 gamma Antibody Featured Recommendations:

1 Publications Citing Use of MCE PI3 Kinase p55 gamma Antibody

Product Detail

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Background

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PI3 Kinase p55 gamma Antibody Related Classifications

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