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P2RY13 Antibody

(Synonyms: GPR86, GPR94, FKSG77, P2RY13, P2Y purinoceptor 13, P2Y13, G-protein coupled receptor 86, G-protein coupled receptor 94)
Customer Review

Based on 1 Customer Validation

P2RY13 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to P2RY13.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ELISA, IF-Tissue

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for P2RY13 Antibody
  • P2RY13 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ELISA 1 Info
 
Dilution Ratio 1:500-1:2000 1:100-1:300 1:200-1:1000 20000

Product Details

Description

P2RY13 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to P2RY13.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40.8 kDa
Species Reactivity Database
Immunogen

Purified recombinant fragment of human P2RY13(aa: 209-258) expressed in E. Coli.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for P2RY13 Antibody
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), A549 (lane 4), Rat brain (lane 5), and Rat spleen (lane 6) using P2RY13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    P2RY13 is a Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_Specificity: Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined.

  • Isoforms & Post-Translational Modification

    P2RY13 has 2 isoforms, Q9BPV8-1: amino acid length is 354, molecular weight is 40789 Da (predicted); Q9BPV8-2: amino acid length is 333, molecular weight is 38410 Da (predicted).P2RY13 存在 2 个异构体，Q9BPV8-1：氨基酸个数为 354 个，分子量为 40789 Da (预测)；Q9BPV8-2：氨基酸个数为 333 个，分子量为 38410 Da (预测)。

  • SwissProt ID

    Q9BPV8

  • Synonyms

    GPR86, GPR94, FKSG77, P2RY13, P2Y purinoceptor 13, P2Y13, G-protein coupled receptor 86, G-protein coupled receptor 94

P2RY13 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), A549 (lane 4), Rat brain (lane 5), and Rat spleen (lane 6) using P2RY13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • P2RY13 Antibody Product Image
    1/16
  • Experimental Validation Results for P2RY13 Antibody
  • P2RY13 Antibody Product Image
  • Experimental Validation Results for P2RY13 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), A549 (lane 4), Rat brain (lane 5), and Rat spleen (lane 6) using P2RY13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for P2RY13 Antibody
  • P2RY13 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), A549 (lane 4), Rat brain (lane 5), and Rat spleen (lane 6) using P2RY13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • P2RY13 Antibody Product Image WB