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ARFRP1 Antibody (YA1639)

(Synonyms: ARP; Arp1; ARL18)
Customer Review

Based on 1 Customer Validation

ARFRP1 Antibody (YA1639) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ARFRP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ARFRP1 Antibody (YA1639)
  • ARFRP1 Antibody (YA1639) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

ARFRP1 Antibody (YA1639) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ARFRP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database

Entrez Gene: 117051 Rat

SwissProt: Q63055 Rat

Immunogen

A synthetic peptide of human ARFRP1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103991

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ARFRP1 Antibody (YA1639)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using ARFRP1/ARP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ARFRP1 is a Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver

  • Subcellular Localization

    Golgi apparatus; Golgi apparatus, trans-Golgi network

  • Expression


    Tissue_specificity:Found in most tissues

  • Isoforms & Post-Translational Modification

    Q13795 has 4 isomers: Q13795-1: 22614 Da (predicted); Q13795-2: 19272 Da (predicted); Q13795-3: 17083 Da (predicted); Q13795-4: 13826 Da (predicted).

  • Subunit

    Interacts with SYS1

  • SwissProt ID

    Q13795

  • Gene ID
    10139 [NCBI]
  • Synonyms

    ARP; Arp1; ARL18

  • Research Field

    Signal Transduction

ARFRP1 Antibody (YA1639) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using ARFRP1/ARP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ARFRP1 Antibody (YA1639) Product Image
    1/16
  • Experimental Validation Results for ARFRP1 Antibody (YA1639)
  • ARFRP1 Antibody (YA1639) Product Image
  • Experimental Validation Results for ARFRP1 Antibody (YA1639)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using ARFRP1/ARP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ARFRP1 Antibody (YA1639)
  • ARFRP1 Antibody (YA1639) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293 (lane 3, 15 μg), and C6 (lane 4, 15 μg) using ARFRP1/ARP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ARFRP1 Antibody (YA1639) Product Image WB