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SH-PTP1 Antibody (YA7491)

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Based on 1 Customer Validation

SH-PTP1 Antibody (YA7491) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to SH-PTP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SH-PTP1 Antibody (YA7491)
  • SH-PTP1 Antibody (YA7491) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:2000-1:10000 1:200-1:1000 1:50-1:200 1:5000-1:20000

Product Details

Description

SH-PTP1 Antibody (YA7491) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to SH-PTP1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SH-PTP1 Antibody (YA7491)
    Western blot analysis of extracts from Jurkat (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Mouse spleen (lane 4(20μg)) 、Rat thymus (lane 5(20μg)) and Rat spleen (lane 6(20μg)) using SH-PTP1 Antibody (HY-P87806) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SH-PTP1 is a Tyrosine phosphatase enzyme that plays important roles in controlling immune signaling pathways and fundamental physiological processes such as hematopoiesis. Dephosphorylates and negatively regulate several receptor tyrosine kinases (RTKs) such as EGFR, PDGFR and FGFR, thereby modulating their signaling activities. When recruited to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors such as immunoglobulin-like transcript 2/LILRB1, programmed cell death protein 1/PDCD1, CD3D, CD22, CLEC12A and other receptors involved in immune regulation, initiates their dephosphorylation and subsequently inhibits downstream signaling events. Modulates the signaling of several cytokine receptors including IL-4 receptor. Additionally, targets multiple cytoplasmic signaling molecules including STING1, LCK or STAT1 among others involved in diverse cellular processes including modulation of T-cell activation or cGAS-STING signaling. Within the nucleus, negatively regulates the activity of some transcription factors such as NFAT5 via direct dephosphorylation. Also acts as a key transcriptional regulator of hepatic gluconeogenesis by controlling recruitment of RNA polymerase II to the PCK1 promoter together with STAT5A[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Expression


    Tissue_Specificity: Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells

  • Isoforms & Post-Translational Modification

    P29350 has four isomers: P29350-1: 67561 Da (predicted); P29350-3: 67719 Da (predicted); P29350-2: 63125 Da (predicted); P29350-4: 70131 Da (predicted).
    Phosphorylated on tyrosine residues丨Ubiquitinated after phosphorylation by TAOK3 (PubMed:38166031)

  • Subunit

    Monomer

  • SwissProt ID

    P29350

SH-PTP1 Antibody (YA7491) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Mouse spleen (lane 4(20μg)) 、Rat thymus (lane 5(20μg)) and Rat spleen (lane 6(20μg)) using SH-PTP1 Antibody (HY-P87806) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SH-PTP1 Antibody (YA7491) Product Image
    1/16
  • Experimental Validation Results for SH-PTP1 Antibody (YA7491)
  • SH-PTP1 Antibody (YA7491) Product Image
  • Experimental Validation Results for SH-PTP1 Antibody (YA7491)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Mouse spleen (lane 4(20μg)) 、Rat thymus (lane 5(20μg)) and Rat spleen (lane 6(20μg)) using SH-PTP1 Antibody (HY-P87806) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SH-PTP1 Antibody (YA7491)
  • SH-PTP1 Antibody (YA7491) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、Raw 264.7 (lane 3(20μg)) 、Mouse spleen (lane 4(20μg)) 、Rat thymus (lane 5(20μg)) and Rat spleen (lane 6(20μg)) using SH-PTP1 Antibody (HY-P87806) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SH-PTP1 Antibody (YA7491) Product Image WB