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HOXB9 Antibody (YA3017)

(Synonyms: Homeobox protein Hox-B9; Hox-2E; Hox-2.5; HOXB9; HOX2E)
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Based on 1 Customer Validation

HOXB9 Antibody (YA3017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HOXB9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HOXB9 Antibody (YA3017)
  • HOXB9 Antibody (YA3017) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50

Product Details

Description

HOXB9 Antibody (YA3017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HOXB9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database

Entrez Gene: 3219 Human

SwissProt: P17482 Human

Immunogen

A synthesized peptide derived from human HOXB9 aa1-52/250.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HOXB9 Antibody (YA3017)
    Western blot analysis of extracts from Hela (lane2, 20μg), HCT116 (lane3, 30μg) and K562 (lane4, 30μg) using HOXB9 Antibody (HY-P83272). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    HOXB9 is a Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis

  • Subcellular Localization

    Nucleus

  • SwissProt ID

    P17482

  • Gene ID
    3219 [NCBI]
  • Synonyms

    Homeobox protein Hox-B9; Hox-2E; Hox-2.5; HOXB9; HOX2E

  • Research Field

    Epigenetics and Nuclear Signaling

HOXB9 Antibody (YA3017) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), HCT116 (lane3, 30μg) and K562 (lane4, 30μg) using HOXB9 Antibody (HY-P83272). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • HOXB9 Antibody (YA3017) Product Image
    1/16
  • Experimental Validation Results for HOXB9 Antibody (YA3017)
  • HOXB9 Antibody (YA3017) Product Image
  • Experimental Validation Results for HOXB9 Antibody (YA3017)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), HCT116 (lane3, 30μg) and K562 (lane4, 30μg) using HOXB9 Antibody (HY-P83272). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HOXB9 Antibody (YA3017)
  • HOXB9 Antibody (YA3017) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), HCT116 (lane3, 30μg) and K562 (lane4, 30μg) using HOXB9 Antibody (HY-P83272). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • HOXB9 Antibody (YA3017) Product Image WB