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Phospho-STAT3 (Tyr705) Antibody (YA7351)

(Synonyms: APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor)
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Based on 1 Customer Validation

Phospho-STAT3 (Tyr705) Antibody (YA7351) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-STAT3 (Tyr705).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Phospho-STAT3 (Tyr705) Antibody (YA7351) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-STAT3 (Tyr705).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 88 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 88 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
    HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with human IFN-α (HY-P70241) at concentration of 20 and 50 nM for 30 mins (Serum free). Phospho-STAT3 (Tyr705) protein expression was subsequently analyzed by Western blot. The results demonstrated that IFN-α induced phosphorylation of STAT3 (Tyr705). Primary antibodies used: Phospho-STAT3 (Tyr705) Antibody (HY-P87666) and GAPDH Antibody (HY-P80954A).
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HeLa (lane 3, IFN-α (HY-P70241), 20 ng/mL, 30 mins, serum-starved) and HeLa (lane 4, IFN-α (HY-P70241), 50 ng/mL, 30 mins, serum-starved) using Phospho-STAT3 (Tyr705) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    STAT3 is a widely expressed STAT family transcription factor that regulates cellular growth, differentiation, survival, immunity, hematopoiesis, and cell migration[1]. Mechanistically, cytokines and growth factors activate JAKs, phosphorylate STAT proteins, promote STAT dimerization, and drive nuclear transcriptional regulation[2]. In inflammatory disease models, STAT3 shows strong tyrosine phosphorylation in human ulcerative colitis, Crohn’s disease, and DSS-induced mouse colitis, where IL-6 deficiency reduces colitis and STAT3 activation[3]. In cancer models, persistent STAT3 activation supports proliferation, survival, invasion, metastasis, immune evasion, and drug resistance, making STAT3 signaling a practical axis for tumor biology research[1][4]. Compared with related isoforms, STAT1 knockout mice mainly show impaired interferon signaling, while STAT3 is emphasized in IL-6-linked inflammation, tumor progression, and Th17-related immune dysfunction[2][5]. For experimental applications, JAK/STAT inhibitors such as AG490 and AZD1480 reduce STAT1/STAT3 activation in renal ischemia-reperfusion and α-synuclein neuroinflammation models[6][7].

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Expression


    Tissue_Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells (PubMed:31899195)

  • Isoforms & Post-Translational Modification

    P40763 has three isomers: P40763-1: 88068 Da (predicted); P40763-2: 87981 Da (predicted); P40763-3: 83126 Da (predicted).
    Tyrosine phosphorylated upon stimulation with EGF丨Acetylated on lysine residues by EP300/p300, promoting its activation (PubMed:15653507, PubMed:16285960, PubMed:18782771)丨Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600)丨(Microbial infection) Phosphorylated on Tyr-705 in the presence of S

  • Subunit

    Forms a homodimer or a heterodimer with a related family member (at least STAT1) (PubMed:15653507, PubMed:28065600)

  • SwissProt ID

    P40763

  • Synonyms

    APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

References

[1]. Liu Y, et al. STAT3 and its targeting inhibitors in osteosarcoma. Cell Prolif. 2021 Feb;54(2):e12974. [Content Brief]

[2]. Heim MH. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):75-120. doi: 10.3109/10799899909036638. PMID: 10071751. et al. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):75-120. [Content Brief]

[3]. Suzuki A, et al. CIS3/SOCS3/SSI3 plays a negative regulatory role in STAT3 activation and intestinal inflammation. J Exp Med. 2001 Feb 19;193(4):471-81. [Content Brief]

[4]. Wu CJ, et al. Activation of STAT3 and STAT5 Signaling in Epithelial Ovarian Cancer Progression: Mechanism and Therapeutic Opportunity. Cancers (Basel). 2019 Dec 19;12(1):24. [Content Brief]

[5]. Tolomeo M, et al. The STAT Signaling Pathway in HIV-1 Infection: Roles and Dysregulation. Int J Mol Sci. 2025 Sep 18;26(18):9123. [Content Brief]

[6]. Si Y, et al. Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the JAK/STAT signaling activation. J Transl Med. 2013 Jun 9;11:141. [Content Brief]

[7]. Qin H, et al. Inhibition of the JAK/STAT Pathway Protects Against α-Synuclein-Induced Neuroinflammation and Dopaminergic Neurodegeneration. J Neurosci. 2016 May 4;36(18):5144-59. [Content Brief]

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Phospho-STAT3 (Tyr705) Antibody (YA7351) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with human IFN-α (HY-P70241) at concentration of 20 and 50 nM for 30 mins (Serum free). Phospho-STAT3 (Tyr705) protein expression was subsequently analyzed by Western blot. The results demonstrated that IFN-α induced phosphorylation of STAT3 (Tyr705). Primary antibodies used: Phospho-STAT3 (Tyr705) Antibody (HY-P87666) and GAPDH Antibody (HY-P80954A).

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HeLa (lane 3, IFN-α (HY-P70241), 20 ng/mL, 30 mins, serum-starved) and HeLa (lane 4, IFN-α (HY-P70241), 50 ng/mL, 30 mins, serum-starved) using Phospho-STAT3 (Tyr705) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image
    1/16
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
    1/1
    MedChemExpress Validation
     HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with human IFN-α (HY-P70241) at concentration of 20 and 50 nM for 30 mins (Serum free). Phospho-STAT3 (Tyr705) protein expression was subsequently analyzed by Western blot. The results demonstrated that IFN-α induced phosphorylation of STAT3 (Tyr705). Primary antibodies used: Phospho-STAT3 (Tyr705) Antibody (HY-P87666) and GAPDH Antibody (HY-P80954A).
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HeLa (lane 3, IFN-α (HY-P70241), 20 ng/mL, 30 mins, serum-starved) and HeLa (lane 4, IFN-α (HY-P70241), 50 ng/mL, 30 mins, serum-starved) using Phospho-STAT3 (Tyr705) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
  • Experimental Validation Results for Phospho-STAT3 (Tyr705) Antibody (YA7351)
  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image
    MedChemExpress Validation

    HeLa cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with human IFN-α (HY-P70241) at concentration of 20 and 50 nM for 30 mins (Serum free). Phospho-STAT3 (Tyr705) protein expression was subsequently analyzed by Western blot. The results demonstrated that IFN-α induced phosphorylation of STAT3 (Tyr705). Primary antibodies used: Phospho-STAT3 (Tyr705) Antibody (HY-P87666) and GAPDH Antibody (HY-P80954A).

  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HeLa (lane 3, IFN-α (HY-P70241), 20 ng/mL, 30 mins, serum-starved) and HeLa (lane 4, IFN-α (HY-P70241), 50 ng/mL, 30 mins, serum-starved) using Phospho-STAT3 (Tyr705) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image WB
  • Phospho-STAT3 (Tyr705) Antibody (YA7351) Product Image WB